利用代谢工程大肠杆菌从掌状海带水解物中高效生产琥珀酸。
Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
出版信息
Bioresour Technol. 2016 Aug;214:653-659. doi: 10.1016/j.biortech.2016.04.117. Epub 2016 May 10.
Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20±0.02mol/mol) than glucose (0.48±0.03mol/mol). The concentration and molar yield of succinic acid were 22.40±0.12g/L and 1.13±0.02mol/mol total sugar respectively after 72h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57±0.17g/L) and galactose (18.03±0.10g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production.
琥珀酸是一种 C4 二羧酸，广泛应用于食品、农业、制药和聚合物等领域。本研究首次探索了利用掌状红皮藻生产琥珀酸。在工程化的大肠杆菌 KLPPP 中，敲除了乳酸脱氢酶、丙酮酸甲酸裂解酶、磷酸转乙酰酶-乙酰激酶和丙酮酸氧化酶基因，同时过表达了磷酸烯醇丙酮酸羧激酶。与葡萄糖（0.48±0.03mol/mol）相比，重组菌在半乳糖上表现出更高的琥珀酸理论摩尔产率（1.20±0.02mol/mol）。经过 72 小时的两相发酵，从由葡萄糖（12.57±0.17g/L）和半乳糖（18.03±0.10g/L）组成的掌状红皮藻水解液中得到了 22.40±0.12g/L 的琥珀酸浓度和 1.13±0.02mol/mol 的总糖理论摩尔产率。结果表明，掌状红皮藻大型红藻生物质是生物炼制生产的一种新型、经济替代的原料。
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