Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Int J Lab Hematol. 2019 Jun;41(3):331-337. doi: 10.1111/ijlh.12979. Epub 2019 Feb 20.
Reference intervals for pediatric laboratory tests need to be interpreted in the context of age- and sex-dependent dynamics. However, few reference intervals for healthy ethnic Han Chinese children have previously attempted to establish using large sample sizes. As such, there are no national hematological standards in China for pediatric reference intervals.
We used a direct method to enroll a total of 2164 healthy 1- to 7-year-old children from Henan province. Hematological reference intervals were established by analyzing venous blood sample data on 17 hematologic analytes. The reference values for different ages and sexes were estimated using both parametric methods (mean ± 2 SD) and nonparametric methods (2.5-97.5th percentiles).
We provided reference intervals for 17 hematologic analytes including red blood count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and red cell distribution width, white blood count and differential count as well as platelet count and related parameters.
We established age- and sex-specific reference intervals that can provide more evidence-based guidance for the diagnosis and treatment for pediatric diseases. Our findings provide the basis for the next step in establishing national blood testing standards.
儿科实验室检测的参考区间需要结合年龄和性别相关的动态变化来进行解读。然而，此前很少有研究试图使用大样本量来建立健康汉族儿童的参考区间。因此，中国没有针对儿科参考区间的全国血液学标准。
我们采用直接方法，共招募了来自河南省的 2164 名 1-7 岁健康儿童。通过分析 17 项血液分析物的静脉血样数据，建立了血液学参考区间。使用参数方法（均值±2SD）和非参数方法（2.5-97.5 百分位数）来估计不同年龄和性别的参考值。
我们提供了 17 项血液分析物的参考区间，包括红细胞计数、血红蛋白浓度、血细胞比容、平均红细胞体积、平均红细胞血红蛋白、平均红细胞血红蛋白浓度和红细胞分布宽度、白细胞计数和分类计数以及血小板计数和相关参数。
我们建立了年龄和性别特异性的参考区间，为儿科疾病的诊断和治疗提供了更具证据的指导。我们的研究结果为下一步建立全国血液检测标准奠定了基础。
