Centre National de Formation et de Recherche en Santé Rurale de Maferinyah, Forecariah, Guinea.
Bioclinical and Fundamental Sciences Chair, Department of Medical Sciences, Faculty of Health Science and Techniques, Gamal Abdel Nasser University of Conakry, Conakry, Guinea.
Biomed Res Int. 2020 Aug 3;2020:8605485. doi: 10.1155/2020/8605485. eCollection 2020.
Guinea's reference ranges for biological parameters rely on those of Caucasian values. Variability in reference ranges according to the context is well-documented. We conducted this study for the purpose of future malaria clinical trials that assess the efficacy and safety of malaria drugs. A repeated cross-sectional study was carried out, in an apparently healthy cohort population. Surveys took place in Maferinyah rural community, which is located at 75 km from the capital. The 2.5 and 97.5 percentiles were determined nonparametrically and stood for reference intervals. Reference values were determined separately for males and females according to ranges of age (6-10 years of age; 11-15 years of age; 16-45 years of age). Differences between genders were tested using the Mann-Whitney test, while the Friedman test was performed to test differences within each gender group according to the seasons. A total of 450 volunteers were enrolled. The median age was 13. Males 16-45 years of age had significantly higher hematologic and biochemical values compared to a female of the same age (for hematological parameters: Mean Cell Hemoglobin Concentration MCHC ≤ 0.001, Platelets ≤ 0.001, monocytes = 0.0305, eosinophils = 0.0225; for biochemical parameters: Aspartate aminotransferase AST ≤ 0.001, Alanine Aminotransferase ALT ≤ 0.001, creatinine ≤ 0.001). We noticed significant seasonal variations for all the biochemical parameters and some hematologic parameters (Mean Corpuscular Hemoglobin MCH, MCHC, Mean Cell volume). This is the first study establishing hematologic and biochemical parameters in Guinea. These findings provide a useful guide for the clinical researchers and care providers. Studies on large scale and in different settings would be also desirable.
几内亚的生物学参数参考范围依赖于白种人数值。参考范围因环境而异的可变性已有充分记录。我们进行这项研究是为了未来评估抗疟药物疗效和安全性的疟疾临床试验。这是一项在显然健康的队列人群中进行的重复横断面研究。调查在距离首都 75 公里的 Maferinyah 农村社区进行。2.5 和 97.5 百分位数采用非参数方法确定，代表参考区间。根据年龄范围（6-10 岁；11-15 岁；16-45 岁），分别为男性和女性确定参考值。使用 Mann-Whitney 检验测试性别间差异，而使用 Friedman 检验根据季节测试每个性别组内的差异。共纳入 450 名志愿者。中位数年龄为 13 岁。16-45 岁的男性与同年龄段的女性相比，血液学和生化值显著更高（血液学参数：平均细胞血红蛋白浓度 MCHC ≤ 0.001，血小板 ≤ 0.001，单核细胞 = 0.0305，嗜酸性粒细胞 = 0.0225；生化参数：天门冬氨酸氨基转移酶 AST ≤ 0.001，丙氨酸氨基转移酶 ALT ≤ 0.001，肌酐 ≤ 0.001）。我们注意到所有生化参数和一些血液学参数（平均红细胞血红蛋白 MCH、MCHC、平均红细胞体积）都有显著的季节性变化。这是在几内亚首次建立血液学和生化学参数的研究。这些发现为临床研究人员和护理提供者提供了有用的指南。还需要在更大规模和不同环境中进行研究。
