Clin Lab. 2020 Apr 1;66(4). doi: 10.7754/Clin.Lab.2019.190729.
Hematological reference interval is the range between two reference values that are used for inter-pretation of test results. It is affected by various physiological and environmental factors; thus, locally derived hematological reference values are essential for accurate diagnosis and treatment of patients. The main goal of this study was to establish hematological reference intervals for healthy adults at Kemise, Northeast Ethiopia.
A cross-sectional study was conducted from January to April, 2019, with 170 male and 159 female apparently healthy adult blood donors at Kemise Blood Bank. A structured pretested questionnaire was used for socio demographic and clinical data collection. About 4 mL of blood was collected in an EDTA test tube and analyzed using Sysmex XP-300 to enumerate the hematological parameters. The data were collected and entered into Epi-Inf7 and then transferred to SPSS version 20 for analysis. Dixon and Reed 1/3 rule was used for outlier detection. Mann-Whitney U test was used to determine reference intervals. Harris and Boyd test were used to determine the reference intervals that need partition. The 2.5th and 97.5th percentiles were determined non-parametrically with 95% confidence interval.
The 2.5th - 97.5th percentiles reference intervals for red blood cell males: 3.9 - 6 x 1012/L, females: 3.3 - 5.3 x 1012/L; hemoglobin males: 12 - 17.9 g/dL, females: 10.5 - 16.3 g/dL; and hematocrit males: 34.8 - 51.4%, females: 30.2 - 47%. The combined reference intervals for both genders, were mean corpuscular volume: 77.1 - 95.5 fL, mean corpuscular hemoglobin: 25.6 - 34 pg, mean corpuscular hemoglobin concentration: 31.5 - 36.7 g/dL, red cell distribution width: 11.3 - 15.4%, white blood cells: 3.1 - 10.6 x 109/L, lymphocytes: 1 - 3.7 x 109/L, mixed: 0.3 - 1.7 x 109/L, neutrophils: 1.4 - 7 x 109/L, and platelets: 136 - 425 x 109/L.
The established reference intervals in this study were different from previous studies which were conducted in different regions of Ethiopia or African countries or in Caucasian population, and in text books. The RBC, PCV, and Hgb reference intervals were different in gender. So, using of locally determined reference interval is essential.
血液学参考区间是用于解释检测结果的两个参考值之间的范围。它受到各种生理和环境因素的影响；因此，为了准确诊断和治疗患者，获得当地的血液学参考值是必不可少的。本研究的主要目的是建立埃塞俄比亚东北部 Kemise 地区健康成年人的血液学参考区间。
2019 年 1 月至 4 月期间，采用横断面研究方法，在 Kemise 血库招募了 170 名男性和 159 名女性健康成年献血者。使用结构化的预测试问卷收集社会人口统计学和临床数据。采集约 4mL 的 EDTA 试管血样，使用 Sysmex XP-300 进行血液学参数计数。数据收集并输入 Epi-Inf7 后转移到 SPSS 20 进行分析。采用 Dixon 和 Reed 1/3 规则检测异常值。采用 Mann-Whitney U 检验确定参考区间。采用 Harris 和 Boyd 检验确定需要分区的参考区间。采用非参数方法确定 2.5%和 97.5%百分位数，置信区间为 95%。
男性红细胞参考区间 2.5% - 97.5%：3.9 - 6 x 1012/L，女性：3.3 - 5.3 x 1012/L；血红蛋白男性：12 - 17.9 g/dL，女性：10.5 - 16.3 g/dL；血细胞比容男性：34.8 - 51.4%，女性：30.2 - 47%。男女混合参考区间：平均红细胞体积：77.1 - 95.5 fL，平均红细胞血红蛋白：25.6 - 34 pg，平均红细胞血红蛋白浓度：31.5 - 36.7 g/dL，红细胞分布宽度：11.3 - 15.4%，白细胞：3.1 - 10.6 x 109/L，淋巴细胞：1 - 3.7 x 109/L，混合细胞：0.3 - 1.7 x 109/L，中性粒细胞：1.4 - 7 x 109/L，血小板：136 - 425 x 109/L。
本研究建立的参考区间与在埃塞俄比亚或非洲国家或白种人群中进行的先前研究以及教科书中的参考区间不同。红细胞、PCV 和 Hgb 参考区间在性别上存在差异。因此，使用当地确定的参考区间是必要的。
