Murphy J F, Zitter T A, Erb A
Department of Entomology and Plant Pathology, Auburn University, AL 36849.
Department of Plant Pathology, Cornell University, Ithaca, NY 14853.
Plant Dis. 2003 Feb;87(2):202. doi: 10.1094/PDIS.2003.87.2.202D.
Jalapeno pepper plants (Capsicum annuum cv. Jaladuro) grown in Erie County, New York expressed chlorotic oak-leaf patterns along the primary vein of fully expanded leaves. Fruit had patterns of irregular ripening with a bumpy surface. Symptom expression was most obvious in August 2002, when leaf and fruit abscission occurred. Symptomatic fruit samples were tested by western blot analysis for the presence of Cucumber mosaic virus (CMV), Potato virus Y (PVY), Pepper mottle virus (PepMoV), Tobacco etch virus (TEV), and Tobacco mosaic virus (TMV). A positive reaction for TMV, but none of the other viruses, was observed. Symptomatic leaf samples were tested by Agdia, Inc. (Elkhart, IN) for Alfalfa mosaic virus, CMV, Impatiens necrotic spot virus, Pepper mild mottle virus, PepMoV, PVY, TEV, TMV, Tobacco ringspot virus, Tomato ringspot virus, and TSWV and for potyviruses using a group-specific test. The Agdia test confirmed that the pepper plants were infected with TMV. The pepper field where the original samples were collected was surveyed for TMV-infected plants. Fifty symptomatic plants expressing foliar and fruit symptoms similar to those originally tested, and 50 asymptomatic plants were sampled by collection of three leaves per plant and tested using enzyme-linked immunosorbent assay for the presence of TMV. All symptomatic plants and 18% of asymptomatic plants tested positive for TMV. To our knowledge, this is the first occurrence of TMV causing losses in commercially grown pepper in New York.
生长在纽约伊利县的墨西哥胡椒植株（辣椒属cv. Jaladuro品种）在完全展开叶片的主脉处呈现出褪绿的橡树叶图案。果实有不规则成熟的图案，表面凹凸不平。症状表现最明显的时间是2002年8月，当时出现了叶片和果实脱落的情况。对有症状的果实样本进行蛋白质免疫印迹分析，检测黄瓜花叶病毒（CMV）、马铃薯Y病毒（PVY）、辣椒斑驳病毒（PepMoV）、烟草蚀纹病毒（TEV）和烟草花叶病毒（TMV）的存在情况。结果观察到对TMV呈阳性反应，而对其他病毒均无阳性反应。对有症状的叶片样本由Agdia公司（印第安纳州埃尔克哈特）检测苜蓿花叶病毒、CMV、凤仙坏死斑病毒、辣椒轻斑驳病毒、PepMoV、PVY、TEV、TMV、烟草环斑病毒、番茄环斑病毒和番茄斑萎病毒，并使用组特异性检测方法检测马铃薯Y病毒属病毒。Agdia检测证实辣椒植株感染了TMV。对采集原始样本的辣椒田进行TMV感染植株调查。采集50株表现出与最初检测的植株相似的叶部和果实症状的有症状植株以及50株无症状植株，每株采集三片叶子进行采样，并使用酶联免疫吸附测定法检测TMV的存在情况。所有有症状植株以及18%的无症状植株TMV检测呈阳性。据我们所知，这是TMV首次在纽约商业化种植的辣椒中造成损失。
