Kosaka Yoshitaka, Ryang Bo-Song, Kobori Takashi, Shiomi Hiroshi, Yasuhara Hisao, Kataoka Mitsunobu
Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto 619-0244.
Laboratory of Plant Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka 599-8531.
Plant Dis. 2006 Jan;90(1):67-72. doi: 10.1094/PD-90-0067.
To cross-protect cucumber plants from Zucchini yellow mosaic virus (ZYMV), we used cold treatment to obtain an attenuated isolate of ZYMV, designated ZYMV-2002. ZYMV-2002 was obtained from a virulent ZYMV isolate after repeated low temperature treatment at 12.5 to 15°C followed by five cycles of single-plant transfer. The isolate produced very mild or no symptoms on cucurbit plants. In addition, inoculated cucumber plants had very similar fruit productivity to healthy control plants under field conditions. During field experiments in 2002 and 2003, when other viruses were also present, protected plants significantly suppressed infection with ZYMV, progression of disease severity, and reduction of fruit yield and quality. These results demonstrate that ZYMV-2002 is a potentially useful attenuated ZYMV isolate for reducing the impact of ZYMV.
为了使黄瓜植株对小西葫芦黄花叶病毒（ZYMV）产生交叉保护，我们采用冷处理获得了一株ZYMV减毒株，命名为ZYMV - 2002。ZYMV - 2002是通过将一株强毒ZYMV分离株在12.5至15°C下反复低温处理，随后进行五个单株转接循环而获得的。该分离株在葫芦科植物上产生非常轻微的症状或无症状。此外，在田间条件下，接种的黄瓜植株与健康对照植株的果实产量非常相似。在2002年和2003年的田间试验中，当也存在其他病毒时，受保护的植株显著抑制了ZYMV的感染、病情严重程度的发展以及果实产量和品质的下降。这些结果表明，ZYMV - 2002是一种潜在有用的ZYMV减毒株，可用于减轻ZYMV的影响。
