Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China; Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.
Fish Shellfish Immunol. 2023 Oct;141:109052. doi: 10.1016/j.fsi.2023.109052. Epub 2023 Sep 5.
MicroRNAs (miRNAs) are a group of RNAs that regulate gene expression in the post-transcriptionally. miRNAs can regulate numerous processes, such as the immune response, due to their dynamic expression patterns. The giant freshwater prawn Macrobrachium rosenbergii is a major freshwater aquaculture prawn that is attacked by various bacteria, including Aeromonas hydrophila. For this study, we performed an analysis of the miRNA and mRNA transcriptome analysis of M. rosenbergii which was infected with A. hydrophila. We identified 56 differentially expressed miRNAs (DEMs) and 1542 differentially expressed mRNAs. Furthermore, an integrated analysis of miRNA-mRNA expression led to the identification of 729 differentially predicted target genes (DETGs) of the DEMs. Multiple functional categories related to immunity, apoptosis, and autophagy were found to be enriched in the DETGs. During the infection of M. rosenbergii by A. hydrophila, an elaborate regulatory network involving Toll and immune deficiency (IMD) signaling, mitogen-activated protein kinase (MAPK) signaling, lysosome, and cell apoptosis was formed by a complex interplay of 40 crucial DEMs and 22 DETGs, all associated with the immune and autophagy pathway. The findings suggest that infection with A. hydrophila triggers intricate responses in both miRNA and mRNA, significantly impacting immune and autophagy processes in M. rosenbergii.
微小 RNA（miRNAs）是一组在后转录水平上调节基因表达的 RNA。由于其动态表达模式，miRNAs 可以调节许多过程，如免疫反应。巨型淡水虾罗氏沼虾是一种主要的淡水养殖虾，受到包括嗜水气单胞菌在内的各种细菌的攻击。在这项研究中，我们对感染嗜水气单胞菌的罗氏沼虾进行了 miRNA 和 mRNA 转录组分析。我们鉴定了 56 个差异表达的 miRNAs（DEMs）和 1542 个差异表达的 mRNAs。此外，miRNA-mRNA 表达的综合分析导致鉴定出 729 个 DEMs 的差异预测靶基因（DETGs）。在 DETGs 中发现了与免疫、细胞凋亡和自噬相关的多个功能类别被富集。在罗氏沼虾感染嗜水气单胞菌的过程中，40 个关键的 DEMs 和 22 个 DETGs 通过 Toll 和免疫缺陷（IMD）信号、丝裂原激活蛋白激酶（MAPK）信号、溶酶体和细胞凋亡等复杂的相互作用，形成了一个复杂的调控网络，与免疫和自噬途径有关。这些发现表明，嗜水气单胞菌感染在 miRNA 和 mRNA 中引发了复杂的反应，显著影响了罗氏沼虾的免疫和自噬过程。
