Xiang Haiyun, Yan Hua, Sun Bingmei, Feng Fuzhong, Chen Ping
Department of Obstetrics and Gynecology, Linyi Central Hospital Linyi 276400, Shandong Province, China.
Department of Peripheral Vascular Disease, Linyi Central Hospital Linyi 276400, Shandong Province, China.
Am J Transl Res. 2019 Jan 15;11(1):463-472. eCollection 2019.
Little is known about the role of long non-coding RNA SNHG7-1 in the development of recurrent spontaneous abortion (RSA). The aim of the present study was to investigate the levels of SNHG7-1 in villi of RSA, and explore its underlying mechanism. The qRT-PCR assay SNHG7-1 showed that the expression level was downregulated in RSA and HTR-8/SVneo cells. In addition, the growth rate of cells transfected with si-SNHG7-1 was significantly decreased compared to that with si-NC, which was reversed by miR-34a targeted with 3'-UTR. Moreover, miR-34a suppressed the expression of WNT1 by binding with the 3'-UTR, which interact with WNT1 to inhibit the proliferation of cells. Furthermore, miR-34a inhibitor rescued the dysregulation of WNT1, p-β-catenin, β-catenin, cyclinD and c-Myc by si-SNHG7A-1. In short, the current study suggests SNHG7-1 plays as an important role in RSA progression targeted by miR-34a via Wnt/β-catenin signaling pathway, providing a novel insight for the pathogenesis and underlying therapeutic target for RSA.
关于长链非编码RNA SNHG7-1在复发性自然流产（RSA）发生发展中的作用知之甚少。本研究旨在检测RSA患者绒毛组织中SNHG7-1的水平，并探讨其潜在机制。qRT-PCR检测SNHG7-1结果显示，RSA患者及HTR-8/SVneo细胞中SNHG7-1表达水平下调。此外，与转染si-NC的细胞相比，转染si-SNHG7-1的细胞生长速率显著降低，而靶向3'-UTR的miR-34a可逆转此现象。此外，miR-34a通过与3'-UTR结合抑制WNT1表达，而WNT1与miR-34a相互作用抑制细胞增殖。此外，miR-34a抑制剂可挽救由si-SNHG7A-1导致的WNT1、p-β-连环蛋白、β-连环蛋白、细胞周期蛋白D和c-Myc的失调。简而言之，本研究表明SNHG7-1通过miR-34a靶向Wnt/β-连环蛋白信号通路在RSA进展中起重要作用，为RSA的发病机制及潜在治疗靶点提供了新的见解。
