Wang Chun-Yan, Wang Shu-Guang, Wang Jia-Li, Zhou Li-Ying, Liu Hong-Jun, Wang Yi-Feng
Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China (mainland).
Department of Obstetrics and Gynecology, Zaozhuang Maternity and Child Health Care Hospital, Zaozhuang, Shandong, China (mainland).
Med Sci Monit. 2016 Oct 2;22:3514-3522. doi: 10.12659/msm.897147.
BACKGROUND The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA). MATERIAL AND METHODS Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 RSA patients as the case group and another 142 normal pregnancy women as the control group. We used denaturing high-performance liquid chromatography (DHPLC) to determine the genotypes and allele frequencies of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A. RESULTS The GG genotype and G allele frequencies of miRNA-27a rs895819 A/G were higher in the case group than in the control group, and the AA genotype and A allele frequencies of Leptin rs7799039 G/A were also higher in the case group than in the control group (all P<0.05). MiRNA-27a rs895819 A/G and Leptin rs7799039 G/A polymorphisms increased the risk of RSA (Exp (B)=2.732, 95% CI=1.6254.596, P=0.000; Exp (B)=4.081, 95% CI=1.8179.164, P=0.001). GG-AA or AG-AA carriers had a higher risk of RSA. The miRNA-27a expression of AA carriers of miRNA-27a rs895819 was lower than that of AG+GG carriers both in the case and control groups (all P=0.024). The plasma leptin concentration of GG carriers was lower than that of GA+AA carriers in the case group (P=0.026). CONCLUSIONS The polymorphisms of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A may contribute to an increased risk of RSA.
背景 本研究旨在探讨miRNA-27a和瘦素基因多态性与复发性自然流产（RSA）风险之间的可能关联。材料与方法 2013年5月至2015年4月期间，在深圳龙华新区中心医院，我们随机招募了138例RSA患者作为病例组，另外142例正常妊娠妇女作为对照组。我们使用变性高效液相色谱法（DHPLC）来确定miRNA-27a rs895819 A/G和瘦素rs7799039 G/A的基因型和等位基因频率。结果 miRNA-27a rs895819 A/G的GG基因型和G等位基因频率在病例组中高于对照组，瘦素rs7799039 G/A的AA基因型和A等位基因频率在病例组中也高于对照组（所有P<0.05）。miRNA-27a rs895819 A/G和瘦素rs7799039 G/A多态性增加了RSA的风险（Exp(B)=2.732，95%CI=1.6254.596，P=0.000；Exp(B)=4.081，95%CI=1.8179.164，P=0.001）。GG-AA或AG-AA携带者患RSA的风险更高。在病例组和对照组中，miRNA-27a rs895819的AA携带者的miRNA-27a表达均低于AG+GG携带者（所有P=0.024）。病例组中GG携带者的血浆瘦素浓度低于GA+AA携带者（P=0.026）。结论 miRNA-27a rs895819 A/G和瘦素rs7799039 G/A的多态性可能导致RSA风险增加。
