Department of Mechanical & Aerospace Engineering and Department of Biomedical, Biological and Chemical Engineering , University of Missouri , Columbia , Missouri 65211 , United States.
The Wallace H. Coulter Department of Biomedical Engineering , Georgia Institute of Technology and Emory University , Atlanta , Georgia 30332 , United States.
Nano Lett. 2019 Mar 13;19(3):2059-2065. doi: 10.1021/acs.nanolett.9b00217. Epub 2019 Feb 22.
Assembling electrospun nanofibers with controlled alignment into three-dimensional (3D), complex, and predesigned shapes has proven to be a difficult task for regenerative medicine. Herein, we report a novel approach inspired by solids of revolution that transforms two-dimensional (2D) nanofiber mats of a controlled thickness into once-inaccessible 3D objects with predesigned shapes. The 3D objects are highly porous, consisting of layers of aligned nanofibers separated by gaps ranging from several micrometers to several millimeters. Upon compression, the objects are able to recover their original shapes. The porous objects can serve as scaffolds, guiding the organization of cells and producing highly ordered 3D tissue constructs. Additionally, subcutaneous implantation in rats demonstrates that the 3D objects enable rapid cell penetration, new blood vessel formation, and collagen matrix deposition. This new class of 3D hierarchical nanofiber architectures offers promising advancements in both in vitro engineering of complex 3D tissue constructs/models or organs and in vivo tissue repair and regeneration.
将具有可控取向的静电纺纳米纤维组装成三维（3D）、复杂和预定形状的结构，对于再生医学来说一直是一项艰巨的任务。在此，我们报告了一种受旋转体启发的新方法，该方法可将具有可控厚度的二维（2D）纳米纤维垫转变为以前无法获得的具有预定形状的 3D 物体。3D 物体具有高度多孔性，由排列的纳米纤维层组成，层与层之间的间隔从几微米到几毫米不等。在压缩时，这些物体能够恢复其原始形状。多孔物体可用作支架，引导细胞的组织并产生高度有序的 3D 组织构建体。此外，在大鼠中的皮下植入表明，3D 物体能够实现快速细胞渗透、新血管形成和胶原基质沉积。这种新型 3D 分级纳米纤维结构为体外复杂 3D 组织构建体/模型或器官的工程以及体内组织修复和再生提供了有前途的进展。
