Sun Li, Yuan Zhengwei, Jian Lingyan, Jiang Qinghua, Zhang Siwen, Tan Jichun
Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China.
Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodelling of Liaoning Province, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China.
Evid Based Complement Alternat Med. 2019 Jan 16;2019:8312020. doi: 10.1155/2019/8312020. eCollection 2019.
The modified Bushen Antai recipe (BSAT) is a centuries-old traditional Chinese medicine that we use in our center as a therapy against pregnancy loss. Our study aimed to explore the potential benefit and mechanism of BSAT in pregnant rats with mifepristone-induced pregnancy loss.
The signature compounds of the eight BSAT ingredients were analyzed by high-performance liquid chromatography (HPLC). The BSAT group (n = 8) was treated daily with 6.3 ml/kg BSAT from gestation day (D) 0.5 to 10.5 and once with 1.25 mg/kg mifepristone on D 10.5. Normal saline replaced BSAT in the model group (n = 8), and both BSAT and mifepristone in the control group (n = 8). Morphological and histological analyses were performed on D 13.5.
BSAT contains eight medicinal ingredients including and . The HPLC analysis detected the signature compounds of seven medicinal ingredients in the extract. Embryo resorption rate in the BSAT group was significantly lower than that in the model group, although the number of surviving embryos was similar between the two groups. Hematoxylin and eosin (HE) staining suggested that the maximum cross-sectional area of the placenta and the area ratio of the placental labyrinth in the BSAT group were higher than those in the model group. Immunohistochemical (IHC) staining indicated that the expression of ki67, estrogen receptor alpha (ER), and progesterone receptor (PR) in the placental labyrinth of the BSAT group was higher than that of the model group. Furthermore, the protein levels of ER, PR, phospho-Akt/Akt, and phospho-Erk1/2/Erk1/2 in the BSAT group were higher than those in the control group. The mRNA levels of ER and PR in the BSAT group were higher than those in the control group.
BSAT may induce estrogen and progesterone receptors by phosphorylation via the classic Akt and Erk1/2 signaling pathways in the maternal-fetal interface of pregnant rats, thereby reducing the pregnancy loss rate and improving the live birth rate.
改良补肾安胎方（BSAT）是一种传承数百年的传统中药，我们中心将其用于治疗流产。本研究旨在探讨BSAT对米非司酮诱导流产的孕鼠的潜在益处及作用机制。
采用高效液相色谱法（HPLC）分析BSAT八味药材的标志性成分。BSAT组（n = 8）自妊娠第0.5天至10.5天每天给予6.3 ml/kg BSAT，并于第10.5天给予1次1.25 mg/kg米非司酮。模型组（n = 8）用生理盐水替代BSAT，对照组（n = 8）既给予BSAT也给予米非司酮。在第13.5天进行形态学和组织学分析。
BSAT含有包括[具体成分缺失]等八味药材。HPLC分析在提取物中检测到七种药材的标志性成分。BSAT组的胚胎吸收率显著低于模型组，尽管两组存活胚胎数量相似。苏木精-伊红（HE）染色显示，BSAT组胎盘的最大横截面积和胎盘迷路的面积比高于模型组。免疫组织化学（IHC）染色表明，BSAT组胎盘迷路中ki67、雌激素受体α（ER）和孕激素受体（PR）的表达高于模型组。此外，BSAT组中ER、PR、磷酸化Akt/Akt和磷酸化Erk1/2/Erk1/2的蛋白水平高于对照组。BSAT组中ER和PR的mRNA水平高于对照组。
BSAT可能通过经典的Akt和Erk1/2信号通路在孕鼠母胎界面磷酸化诱导雌激素和孕激素受体，从而降低流产率并提高活产率。
