Cakmak Gamze, Cetin Ragbetli Murat
Faculty of Veterinary Medicine, Department of Anatomy, University of Yuzuncu Yil, Van, Turkey.
Faculty of Medicine, Department of Histology and Embryology, University of Yuzuncu Yil, Van, Turkey.
Anat Histol Embryol. 2020 Jan;49(1):17-24. doi: 10.1111/ahe.12435. Epub 2019 Feb 21.
Stereology is a method for examining two-dimensional objects as three-dimensional objects. The aim of this study was to offer volume values for thoracic segments in ducks by means of stereological methods. This study examined the data obtained from stereological analysis of the total volume and grey and white matter volume values of the thoracic segment, a part of the adult duck spinal cord with a weight of 3-4 kg. In the study, study samples consisted of 10 adult ducks (Anas) used without gender discrimination. To perfuse all of the animals, 10% formaldehyde was utilised. The perfused animals were kept in 10% formaldehyde for one week. The spinal cord was uncovered following the removal of arcus vertebrae parts of thoracic vertebrae in the thoracic part of dissected ducks. Tissue samples of thoracic segments were taken; 5-µm-thick cross sections from these tissue samples were taken via microtome. Attention was paid to obtaining samples at the ratio of 1/250 by taking 12 cross sections from each segment. The cross sections were subjected to haematoxylin-eosin staining. Photographs of all cross sections were taken using a microscope. The volume values of all tissue and grey and white matter structures in each thoracic segment of the spinal cord were calculated. The total volume, grey and white matter volume densities in thoracic segments of ducks, as well as the data obtained as a result of proportioning volume values of one another are represented in the conclusion section of this study.
体视学是一种将二维物体视为三维物体进行检查的方法。本研究的目的是通过体视学方法提供鸭胸段的体积值。本研究检查了从成年鸭脊髓一部分胸段的总体积以及灰质和白质体积值的体视学分析中获得的数据，该成年鸭体重为3 - 4千克。在该研究中，研究样本由10只成年鸭（鸭属）组成，使用时未区分性别。为灌注所有动物，使用了10%的甲醛。灌注后的动物在10%甲醛中保存一周。在解剖鸭的胸部去除胸椎的椎弓部分后露出脊髓。采集胸段的组织样本；通过切片机从这些组织样本中获取5微米厚的横截面。注意通过从每个节段取12个横截面以1/250的比例获取样本。对横截面进行苏木精 - 伊红染色。使用显微镜拍摄所有横截面的照片。计算脊髓每个胸段中所有组织以及灰质和白质结构的体积值。鸭胸段的总体积、灰质和白质体积密度，以及通过相互比例化体积值获得的数据在本研究的结论部分呈现。
