Cakmak Gamze, Karadag Huseyin
Faculty of Veterinary Medicine, Department of Anatomy, University of Van Yuzuncu Yil, Van, Turkey.
Anat Histol Embryol. 2019 Mar;48(2):164-174. doi: 10.1111/ahe.12437. Epub 2019 Mar 5.
In the study, total segment, grey matter and white matter volume values of lumbosacral segment of spinal cord in quails (Coturnix coturnix japonica) were stereologically examined. Regardless of sex, six adult quails were used as material. After the materials were perfused, they were dissected and their spinal cords were uncovered. Tissue samples were taken from each lumbosacral segment of spinal cord. After the tissue fixation process, 6-7 sections having a 5-µm thickness were separated in every 50th section as from the first section, where the tissue was seen, among tissue samples of each segment. These segments were stained with haematoxylin-eosin staining. They were photographed via a microscope. By using the Cavalieri's principle, the volume values of both the total segment, grey matter (GM) and white matter (WM) in each lumbosacral segment were separately calculated. All these calculations were performed by using SHTEREOM 1.5 program. As a result, total volume, grey matter and white matter volume values of lumbosacral segments and white matter/total volume, grey matter/total volume, and grey matter/white matter volume fractions were obtained.
在该研究中，对鹌鹑（日本鹌鹑）脊髓腰骶段的全段、灰质和白质体积值进行了体视学检查。不论性别，选用6只成年鹌鹑作为材料。材料灌注后进行解剖，暴露脊髓。从脊髓的每个腰骶段采集组织样本。组织固定后，在每个节段的组织样本中，从可见组织的第一节开始，每隔50节分离出6 - 7个厚度为5微米的切片。这些切片用苏木精 - 伊红染色。通过显微镜拍照。利用卡瓦列里原理，分别计算每个腰骶段的全段、灰质（GM）和白质（WM）的体积值。所有这些计算均使用SHTEREOM 1.5程序进行。结果，获得了腰骶段的总体积、灰质和白质体积值以及白质/总体积、灰质/总体积和灰质/白质体积分数。
