[Susceptibility status of and to Organophosphates and Carbamates in the Agricultural Exploitation Zones of oil Palm at Mouila, Gabon].

This study was conducted during the rainy season of 2017 in the agricultural areas at Mouila, with the aim to assess the determination of susceptibility of complex to insecticides and the detection of Ace 1 mutation. Mosquito larvae were collected by using the method and nursed to adult stage. The susceptibility of adult populations to organophosphates and carbamates was assessed using the standard WHO susceptibility test protocol. The tested mosquitoes species were identified by PCR. These tested mosquitoes were used to search for the Ace 1 mutation. The insecticides used were pirimiphos-methyl 0.25% and 1.25%, fenitrothion 1%, malathion 5%, propoxur 0.1%, bendiocarb 0.1%, 0.5% and 1%. In the prospected areas, included An. gambiae s.s. and sympatric in their larval habitats. However, An. gambiae s.s. was predominant in all studied areas (99.1%) comparatively to Moreover, the susceptibility tests of Anopheles to organophosphates and carbamates revealed mortality rates of 100% regardless of the origin of the mosquitoes. PCR diagnosis of Ace 1 mutation showed that none tested mosquito (An. gambiae s.s. and ) from prospected areas harbored the Ace 1 gene in all prospected areas. The lack of Ace 1 mutation in reveals the current effectiveness of organophosphates and carbamates for the control of these mosquitoes. Bboth these classes of insecticides could be used for the anopheline populations control in the agricultural spaces at Mouila.