CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics , Chinese Academy of Sciences , Lanzhou 730000 , P. R. China.
University of Chinese Academy of Sciences , Beijing 100049 , P. R. China.
Langmuir. 2019 Apr 2;35(13):4471-4480. doi: 10.1021/acs.langmuir.8b03997. Epub 2019 Mar 25.
Macroporous adsorption resins (MARs) have experienced rapid growth because of their unique properties and applications. Recently, it was discovered that a series of MARs with super-macroporous and diverse functional groups were synthesized to adsorb and enrich peptides; however, the detailed change mechanism of pore diameter and element composition and peptide adsorption mechanism have not yet been established. In this study, MARs and modified MARs were prepared by the surfactant reverse micelles swelling method and Friedel-Crafts reaction, and the pore diameter and element changes of these super-macroporous resin particles were accurately determined to elucidate formation processes of modified MARs. The adsorption mechanism of four peptides on different MARs was investigated. Sieving effect, electrostatic, hydrophobic, and hydrogen bonds interactions were found to play a major role in the adsorption process of peptides. Compared to that of the traditional resins, the adsorption capacity of super-macroporous MARs for peptides enormously increased. Electrostatic interactions have been explained perfectly by determining the isoelectric point. The molecular docking technology proved that the hydrogen-bonding receptor in MARs was a crucial factor for the adsorption capacity by autodock 4.26 and gromacs 5.14. These findings will enable selective adsorption of peptides by MARs, which also provides a theoretical basis for the construction of specific resin to adsorb different peptides.
大孔吸附树脂（MARs）因其独特的性质和应用而得到了快速发展。最近，人们发现一系列具有超微孔和多种官能团的 MARs 被合成用于吸附和富集肽；然而，孔径和元素组成的详细变化机制以及肽吸附机制尚未建立。在本研究中，通过表面活性剂反胶束溶胀法和傅克反应制备了 MARs 和改性 MARs，并准确地测定了这些超大孔树脂颗粒的孔径和元素变化，以阐明改性 MARs 的形成过程。研究了四种肽在不同 MARs 上的吸附机制。筛分效应、静电、疏水和氢键相互作用被发现对肽的吸附过程起主要作用。与传统树脂相比，超大孔 MARs 对肽的吸附容量大大增加。通过测定等电点，完美地解释了静电相互作用。分子对接技术证明，MARs 中的氢键受体是吸附容量的关键因素，这是通过 autodock 4.26 和 gromacs 5.14 证明的。这些发现将使 MARs 能够对肽进行选择性吸附，这也为构建特定的树脂以吸附不同的肽提供了理论依据。
