Department of Biochemical Engineering , University College London , London , WC1E 7JE , United Kingdom.
Department of Chemistry, Institute of Structural and Molecular Biology , University College London , London , WC1H 0AJ , United Kingdom.
ACS Nano. 2019 Mar 26;13(3):3334-3340. doi: 10.1021/acsnano.8b09200. Epub 2019 Feb 22.
Nanopores are powerful nanodevices that puncture semifluid membranes to enable transport of molecular matter across biological or synthetic thin layers. Advanced nanopores featuring more complex functions such as ambient sensing and reversible channel opening are of considerable scientific and technological interest but challenging to achieve with classical building materials. Here we exploit the predictable assembly properties of DNA to form a multifunctional nanovalve that senses temperature for controlled channel opening and tunable transport. The barrel-shaped valve is formed from solely seven oligonucleotides and is closed at ambient temperatures. At >40 °C a programmable thermosensitive lid opens the barrel to allow transport of small molecules across the membrane. The multifunctional DNA nanodevice may be used to create logic ionic networks or to achieve controlled drug delivery from vesicles.
纳米孔是强大的纳米器件，能够刺穿半流体膜，使分子物质在生物或合成薄膜中传输。具有更复杂功能的先进纳米孔，如环境感应和可逆通道打开，具有相当大的科学和技术兴趣，但用传统建筑材料很难实现。在这里，我们利用 DNA 的可预测组装特性来形成一种多功能纳米阀，该纳米阀可以感应温度以进行可控的通道打开和可调的传输。桶形阀仅由 7 个寡核苷酸组成，在环境温度下关闭。在 >40°C 时，可编程热敏盖打开桶形阀，允许小分子穿过膜进行传输。这种多功能 DNA 纳米器件可用于构建逻辑离子网络或从囊泡中实现药物的控制释放。
