"Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Bosn J Basic Med Sci. 2019 May 20;19(2):201-209. doi: 10.17305/bjbms.2019.3854.
In vitro studies showed that high-frequency pulsed electromagnetic fields (HF-PEMFs) increase the activity/expression of early and late osteogenic markers and enhance bone mineralization. The main aim of this study was to investigate the in vivo effects of HF-PEMFs on fracture healing using a rat model. A femur fracture was established by surgery in 20 male Wistar rats. Titanium nails were implanted to reduce and stabilize the fracture. After surgery, 20 rats were equally divided into untreated control and treated group (from the first postoperative day HF-PEMFs at 400 pulses/sec [pps] were applied for 10 minutes/day, for two weeks). Quantitative and qualitative assessment of bone formation was made at two and eight weeks following surgery and included morphological and histological analysis, serological analysis by ELISA, micro-computed tomography (micro-CT), and three-point bending test. At two weeks in HF-PEMF group, soft callus was at a more advanced fibrocartilaginous stage and the bone volume/total tissue volume (BV/TV) ratio in the callus area was significantly higher compared to control group (p = 0.047). Serum concentration of alkaline phosphatase (ALP) and osteocalcin (OC) was significantly higher in HF-PEMF group (ALP p = 0.026, OC p = 0.006) as well as the mechanical strength of femurs (p = 0.03). At eight weeks, femurs from HF-PEMF group had a completely formed woven bone with dense trabeculae, active bone marrow, and had a significantly higher BV/TV ratio compared to control (p = 0.01). HF-PEMFs applied from the first postoperative day, 10 minutes/day for two weeks, enhance bone consolidation in rats, especially in the early phase of fracture healing.
体外研究表明，高频脉冲电磁场（HF-PEMFs）可增加早期和晚期成骨标志物的活性/表达，并增强骨矿化。本研究的主要目的是通过大鼠模型研究 HF-PEMFs 对骨折愈合的体内作用。通过手术在 20 只雄性 Wistar 大鼠中建立股骨骨折。植入钛钉以减少和稳定骨折。手术后，将 20 只大鼠等分为未治疗对照组和治疗组（从术后第一天开始，每天应用 400 脉冲/秒 [pps] 的 HF-PEMFs 治疗 10 分钟，持续两周）。术后 2 周和 8 周进行骨形成的定量和定性评估，包括形态学和组织学分析、ELISA 血清分析、微计算机断层扫描（micro-CT）和三点弯曲试验。在 HF-PEMF 组中，在第 2 周时，软痂处于更先进的纤维软骨阶段，骨痂区域的骨体积/总组织体积（BV/TV）比值明显高于对照组（p=0.047）。HF-PEMF 组的血清碱性磷酸酶（ALP）和骨钙素（OC）浓度明显更高（ALP p=0.026，OC p=0.006），股骨的机械强度也更高（p=0.03）。在第 8 周时，HF-PEMF 组的股骨具有完全形成的编织骨，具有密集的小梁，活跃的骨髓，并且与对照组相比，BV/TV 比值明显更高（p=0.01）。从术后第一天开始，每天应用 10 分钟的 HF-PEMFs，持续两周，可增强大鼠的骨整合，尤其是在骨折愈合的早期阶段。
