Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Departamento de Ciências da Saúde, Universidade Federal de Santa Catarina, Araranguá, Brazil.
Physiol Behav. 2019 May 15;204:248-255. doi: 10.1016/j.physbeh.2019.02.023. Epub 2019 Feb 19.
Benefits of exercise have been documented for many diseases with a chronic progression, including obesity, diabetes mellitus, cardiovascular diseases, neurodegenerative diseases, certain types of cancers, and overall mortality. Low-grade systemic inflammation is a key component of these pathologies and it has been demonstrated that can be prevented by performing regularly physical exercise. The aim of this study was to examine the effect of lipopolysaccharide (LPS)-induced inflammation on glucose and insulin tolerance, exercise performance, production of urinary neopterin and striatal neurotransmitters levels in adult male C57BL/6 mice. Increased blood glucose clearance and insulin sensitivity were observed after a single administration of glucose (2 g/kg, p.o.) or insulin (0.5 U/kg, i.p.). However, the repeated injection of LPS (0.33 mg/kg/day, i.p.) decreased glucose tolerance and increase urinary neopterin levels, pointing to systemic inflammation. In parallel to the urinary-increased neopterin, it was observed a significant reduction in the striatal dopamine levels and an increase in the serotonin/dopamine ratio. While a single LPS injection (0.33 mg/kg, i.p.) showed impaired performance in the incremental loading test (10 m/min, with 2 m/min increment every 3 min, at 9% grade), a moderate physical exercise protocol (treadmill for three weeks; 5 sessions/week; up to 50 min/day) prevented the exacerbation of immune system activation and preserved mitochondrial activity in skeletal muscle from mice with continuous LPS infusion (infusion pumps: 0.83 mg/kg/day, i.p.). In conclusion, the peripheral-induced inflammation elicited metabolic alterations that provoked impairment in striatal dopamine metabolism. The moderate exercise prevented the increase of urinary neopterin and preserved mitochondrial activity under LPS-induced inflammatory conditions.
运动对许多慢性进展性疾病都有好处，包括肥胖、糖尿病、心血管疾病、神经退行性疾病、某些类型的癌症和总体死亡率。低度全身炎症是这些病理的一个关键组成部分，已经证明定期进行体育锻炼可以预防这种炎症。本研究旨在研究脂多糖（LPS）诱导的炎症对成年雄性 C57BL/6 小鼠葡萄糖和胰岛素耐量、运动表现、尿中新蝶呤产生和纹状体神经递质水平的影响。单次给予葡萄糖（2 g/kg，口服）或胰岛素（0.5 U/kg，腹腔内注射）后，观察到血糖清除率和胰岛素敏感性增加。然而，重复注射 LPS（0.33 mg/kg/天，腹腔内注射）降低了葡萄糖耐量并增加了尿中新蝶呤水平，提示存在全身炎症。与尿中新蝶呤增加平行，观察到纹状体多巴胺水平显著降低，而血清素/多巴胺比值增加。单次 LPS 注射（0.33 mg/kg，腹腔内注射）显示在递增负荷试验（10 m/min，每 3 分钟增加 2 m/min，在 9%坡度）中表现不佳，而适度的运动方案（跑步机三周；每周 5 次；每天 50 分钟）防止了连续 LPS 输注小鼠免疫系统激活的恶化，并保持了骨骼肌中线粒体的活性（输注泵：0.83 mg/kg/天，腹腔内注射）。总之，外周诱导的炎症引起了代谢改变，导致纹状体多巴胺代谢受损。适度运动可防止 LPS 诱导的炎症状态下尿中新蝶呤的增加，并保持线粒体活性。
