Theilmann J, Orban S, Rochon D
Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97, Summerland, British Columbia V0H 1Z0.
Plant Dis. 2004 Oct;88(10):1092-1098. doi: 10.1094/PDIS.2004.88.10.1092.
The LC5 isolate of Little cherry virus (LChV-LC5) is one of at least two distinct viruses contributing to a severe disease of cherry (Little cherry disease [LChD]) in British Columbia. A near-complete nucleotide sequence of LChV-LC5 is available as well as polyclonal antibodies against LChV-LC5 coat protein produced in bacterial cells. A survey for LChV-LC5-infected trees in the Okanagan Valley and Kootenay region of British Columbia was carried out using enzyme-linked immunosorbent assays (ELISA) and LChV-LC5 antibodies. Reverse transcriptase polymerase chain reaction (RT-PCR) and sequence analysis of four different regions of the genomes of 31 of these isolates have been conducted. A high level of sequence variability was found: nucleotide sequence divergence between LChV-LC5 and the other sequenced isolates ranged from 0 to 19.7%, and amino acid sequence divergence ranged from 0 to 9.1%. Further examination of RT-PCR and sequence data identified six discrete groups of isolates, including a group identical to LChV-LC5. The high level of divergence in LChV-LC5 isolates occurring in British Columbia suggests that caution should be used in the selection of methods used for diagnosis during surveys for this virus.
小樱桃病毒（LChV-LC5）的LC5分离株是导致不列颠哥伦比亚省樱桃严重病害（小樱桃病[LChD]）的至少两种不同病毒之一。已有LChV-LC5的近乎完整的核苷酸序列以及在细菌细胞中产生的针对LChV-LC5外壳蛋白的多克隆抗体。利用酶联免疫吸附测定（ELISA）和LChV-LC5抗体，对不列颠哥伦比亚省奥卡纳根山谷和库特奈地区感染LChV-LC5的树木进行了调查。对其中31个分离株基因组的四个不同区域进行了逆转录酶聚合酶链反应（RT-PCR）和序列分析。发现了高度的序列变异性：LChV-LC5与其他测序分离株之间的核苷酸序列差异范围为0至19.7%，氨基酸序列差异范围为0至9.1%。对RT-PCR和序列数据的进一步检查确定了六个离散的分离株组，包括一个与LChV-LC5相同的组。不列颠哥伦比亚省出现的LChV-LC5分离株的高度差异表明，在对该病毒进行调查期间选择诊断方法时应谨慎。
