Pasquali Matias, Dematheis Flavia, Gilardi Giovanna, Gullino Maria Lodovica, Garibaldi Angelo
Di.Va.P.R.A. and Centre of Competence for the Innovation in the Agro-Environmental Field (AGROINNOVA), via Leonardo da Vinci, 44 10095 Grugliasco (TO), Italy.
Plant Dis. 2005 Mar;89(3):237-240. doi: 10.1094/PD-89-0237.
Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.
尖孢镰刀菌莴苣专化型（Fusarium oxysporum f. sp. lactucae）是莴苣枯萎病的病原菌，在过去10年里已在三大洲被报道。对从意大利、美国、日本和台湾受感染的植株及种子中分离得到的47个菌株进行了致病性和营养体亲和性评估。利用抗氯酸盐、不利用硝酸盐的突变体来确定不同地点分离菌株之间的遗传相关性。采用营养体亲和群（VCG）方法，所有意大利和美国的分离菌株、2型台湾分离菌株以及一个日本1号生理小种被归为主要的VCG 0300。1型台湾分离菌株被归为VCG 0301。文中讨论了关于2001 - 2002年在意大利田间莴苣上引发疫情的尖孢镰刀菌莴苣专化型繁殖体可能通过进口和使用受污染种子传播的假说。
