Di Primo P, Cappelli C
Dipartimento di Agrochimica e Agrobiologia, P.zza San F.sco di Sales 2, 89061 Gallina (RC), Italy.
Dipartimento di Arboricoltura e Protezione delle Piante, Borgo XX Giugno 74, 06121 Perugia, Italy.
Plant Dis. 2000 Jul;84(7):806. doi: 10.1094/PDIS.2000.84.7.806C.
Fusarium corm rot of saffron (Crocus sativus L.), incited by Fusarium oxysporum Schlecht. f. sp. gladioli, causes severe stigma yield losses in L'Aquila Province in Central Italy. Primary symptoms during flowering (October through November) include basal stem rot, yellowing and wilting of shoots, and corm rot. The rapid spread of the disease is apparently caused only by movement of contaminated and/or infected corms (2,3). Ten isolates of F. oxysporum f. sp. gladioli, obtained from infected saffron crops located in the principal areas of saffron production in L'Aquila Province (2,3), were characterized by vegetative compatibility tests. The isolates were placed into vegetative compatibility groups (VCGs) using heterokaryon tests with chlorate-resistant nitrate-nonutilizing (nit) mutants. Based on complementation among these isolates and with a representative isolate of VCG 0340 (4), the tested isolates were assigned to VCG 0340. The fact that all isolates examined in this study belong to a single VCG supports the hypothesis that a clonal population of F. oxysporum f. sp. gladioli is spreading in L'Aquila Province in planting stock. In previous studies, only two Italian isolates of the pathogen obtained from Gladiolus were tested for vegetative compatibility, and both were included in VCG 0343 (1,4). Acquisition of further information on a larger number of isolates obtained from other cropping sites and associated with other hosts may result in a better understanding of the origin and spread of the pathogen in Italy, leading to improved strategies for control of Fusarium corm rot of saffron. References: (1) R. P. Baayen et al. Eur. J. Plant Pathol. 104:887, 1998. (2) C. Cappelli and G. Di Minco. Infitore Fitopatol. 49:27, 1999. (3) C. Cappelli and G. Di Minco. J. Plant Pathol. 80:253, 1998. (4) J. J. Mes et al. Plant Pathol. 43:362, 1994.
由尖孢镰刀菌唐菖蒲专化型（Fusarium oxysporum Schlecht. f. sp. gladioli）引起的藏红花（Crocus sativus L.）镰刀菌球茎腐烂病，在意大利中部阿奎拉省导致柱头产量严重损失。开花期（10月至11月）的主要症状包括基部茎腐、嫩枝黄化和枯萎以及球茎腐烂。该病的快速传播显然仅由受污染和/或感染的球茎移动引起（2,3）。从阿奎拉省藏红花主要产区受感染的藏红花作物中获得了10株尖孢镰刀菌唐菖蒲专化型分离株（2,3），通过营养体亲和性测试对其进行了特征分析。使用对氯酸盐抗性的硝酸盐不利用（nit）突变体进行异核体测试，将这些分离株归入营养体亲和群（VCGs）。根据这些分离株之间以及与VCG 0340的一个代表性分离株的互补性（4），将测试的分离株归入VCG 0340。本研究中检测的所有分离株都属于单一的VCG这一事实支持了以下假设：尖孢镰刀菌唐菖蒲专化型的一个克隆群体正在阿奎拉省的种球中传播。在以前的研究中，仅对从唐菖蒲中获得的该病原菌的两株意大利分离株进行了营养体亲和性测试，且二者都属于VCG 0343（1,4）。获取更多关于从其他种植地点获得的、与其他寄主相关的大量分离株的信息，可能会更好地了解该病原菌在意大利的起源和传播，从而改进藏红花镰刀菌球茎腐烂病的防治策略。参考文献：（1）R. P. Baayen等人，《欧洲植物病理学杂志》104:887，1998年。（2）C. Cappelli和G. Di Minco，《植物病理学》49:27，1999年。（3）C. Cappelli和G. Di Minco，《植物病理学杂志》80:253，1998年。（4）J. J. Mes等人，《植物病理学》43:362，1994年。
