Tzanetakis Ioannis E, Postman Joseph D, Martin Robert R
Department of Botany and Plant Pathology & Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331.
National Clonal Germplasm Repository, USDAARS, Corvallis 97333.
Plant Dis. 2005 Jun;89(6):654-658. doi: 10.1094/PD-89-0654.
Mentha × gracilis 'Variegata', described more than 200 years ago, is still being used as an ornamental. The bright vein-banding symptoms that confer the ornamental value to 'Variegata' clones are graft transmissible and can be eliminated after heat therapy and apical meristem culture. This observation led us to investigate the possibility that symptoms are virus-induced. Double-stranded RNA extracted from a 'Variegata' clone was cloned. One of the viruses identified was a member of the Closteroviridae family. This virus, designated Mint vein-banding associated virus, shares sequence similarities with all three genera of the family, making it an important link among the genera of the Closteroviridae. A detection protocol has been developed that readily detects the virus in other mint clones that exhibit vein-banding symptoms.
薄荷× gracilis‘Variegata’于200多年前被描述，至今仍用作观赏植物。赋予‘Variegata’克隆体观赏价值的亮脉带症状可通过嫁接传播，并且在热处理和茎尖分生组织培养后可以消除。这一观察结果促使我们研究症状是否由病毒引起。从一个‘Variegata’克隆体中提取的双链RNA被克隆。鉴定出的其中一种病毒是长线形病毒科的成员。这种病毒被命名为薄荷脉带相关病毒，与该科的所有三个属都有序列相似性，使其成为长线形病毒科各属之间的重要联系。已经开发出一种检测方法，可以很容易地在其他表现出脉带症状的薄荷克隆体中检测到该病毒。
