Wang Fang, Qi Shuishui, Gao Zhengliang, Akinyemi Ibukun A, Xu Dafeng, Zhou Benguo
Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China.
School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
Arch Virol. 2016 Apr;161(4):1087-90. doi: 10.1007/s00705-015-2739-x. Epub 2016 Jan 21.
The complete genome sequence of a novel virus, provisionally named tobacco virus 1 (TV1), was determined, and this virus was identified in leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic and yellowing symptoms in Anhui Province, China. The genome sequence of TV1 consists of 15,395 nucleotides with 61.6 % nucleotide sequence identity to mint virus 1 (MV1). Its genome organization is similar to that of MV1, containing nine open reading frames (ORFs) that potentially encode proteins with putative functions in virion assembly, cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the heat shock protein 70 homolog (HSP70h) placed TV1 alongside members of the genus Closterovirus in the family Closteroviridae. To our knowledge, this study is the first report of the complete genome sequence of a closterovirus identified in tobacco.
一种新型病毒（暂命名为烟草病毒1，TV1）的全基因组序列已被测定，该病毒是在中国安徽省表现出叶片花叶和黄化症状的烟草（Nicotiana tabacum）叶片中鉴定出来的。TV1的基因组序列由15395个核苷酸组成，与薄荷病毒1（MV1）的核苷酸序列同一性为61.6%。其基因组结构与MV1相似，包含9个开放阅读框（ORF），这些开放阅读框可能编码在病毒粒子组装、细胞间移动和RNA沉默抑制中具有推定功能的蛋白质。对热休克蛋白70同源物（HSP70h）的系统发育分析将TV1与长线形病毒科长线形病毒属的成员归为一类。据我们所知，本研究是在烟草中鉴定出的长线形病毒全基因组序列的首次报道。
