Forgia M, Vallino M, Marra M, Mussano P, Lanteri A P, Accotto G P, Ciuffo M
Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135, Torino, Italy.
Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Via Amendola 165/A, 70126, Bari, Italy.
Arch Virol. 2025 Jan 10;170(2):35. doi: 10.1007/s00705-025-06222-8.
Here, we report the complete genome sequence of a new carlavirus causing mosaic on mint plants in Italy, which we have tentatively named "mint virus C" (MVC). Flexuous particles of around 600 nm were observed using transmission electron microscopy, and next-generation sequencing was performed to determine the nucleotide sequence of the MVC genome, which was found to be 8558 nt long, excluding the poly(A) tail, and shows the typical organization of a carlavirus. The putative proteins encoded by MVC are 44-56% identical to the closest matches in the NCBI database, suggesting that MVC should be considered a member of a new species in the genus Carlavirus. MVC was detected in independent mint samples from different regions of Italy, collected in 2023 and 2024. Two MVC isolates, identified in 2023 (Me1) and in 2024 (Me2), have been included in the Plant Virus Italy (PLAVIT) collection.
在此，我们报告了一种在意大利薄荷植株上引起花叶病的新型香石竹潜隐病毒的全基因组序列，我们暂时将其命名为“薄荷病毒C”（MVC）。使用透射电子显微镜观察到约600 nm长的弯曲病毒粒子，并进行了二代测序以确定MVC基因组的核苷酸序列，发现其长度为8558 nt（不包括poly(A)尾），呈现出香石竹潜隐病毒的典型结构。MVC编码的推定蛋白与NCBI数据库中最相似的序列有44%-56%的同一性，这表明MVC应被视为香石竹潜隐病毒属一个新物种的成员。在2023年和2024年从意大利不同地区采集的独立薄荷样本中检测到了MVC。在2023年鉴定的两个MVC分离株（Me1）和2024年鉴定的（Me2）已被纳入意大利植物病毒（PLAVIT）收藏库。
