Wong T K, Domin B A, Bent P E, Blanton T E, Anderson M W, Philpot R M
Cancer Res. 1986 Feb;46(2):999-1004.
Placental tissues were obtained from Chinese women in Taiwan who had been exposed to contaminated rice oils containing polychlorinated biphenyls and their thermal degradative products. Exposure via the diet occurred 4-5 years prior to pregnancy. Placental microsomal fractions from eight of the nine exposed subjects studied showed marked elevation of benzo(a)pyrene hydroxylation and 7-ethoxyresorufin O-deethylation activities related to control subjects. Placental microsomes from exposed subjects were found to contain a protein that cross-reacted with antibodies raised to rabbit cytochrome P-450 isozyme 6, an isozyme induced by polycyclic aromatic hydrocarbons. This protein was not observed with microsomal samples from control subjects. A significant correlation was found between the relative amounts of the immunoreactive protein and benzo(a)-pyrene hydroxylation and 7-ethoxyresorufin O-deethylation activities. The 7-ethoxyresorufin O-deethylation activities were inhibited by alpha-naphthoflavone, a compound known to inhibit activities of rabbit cytochrome P-450, isozyme 6.
胎盘组织取自台湾地区接触过含多氯联苯及其热降解产物的污染米糠油的中国女性。孕期前4至5年通过饮食接触污染物。在研究的9名接触者中，有8人的胎盘微粒体组分显示，与对照者相比，苯并(a)芘羟化酶和7-乙氧基异吩唑酮O-脱乙基酶活性显著升高。研究发现，接触者的胎盘微粒体含有一种蛋白质，该蛋白质可与针对兔细胞色素P-450同工酶6产生的抗体发生交叉反应，细胞色素P-450同工酶6是一种由多环芳烃诱导产生的同工酶。对照者的微粒体样本中未观察到这种蛋白质。免疫反应性蛋白的相对含量与苯并(a)芘羟化酶和7-乙氧基异吩唑酮O-脱乙基酶活性之间存在显著相关性。7-乙氧基异吩唑酮O-脱乙基酶活性受到α-萘黄酮的抑制，α-萘黄酮是一种已知可抑制兔细胞色素P-450同工酶6活性的化合物。
