Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Marche Polytechnic University, I-60020, Ancona, Italy.
Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Marche Polytechnic University, I-60020, Ancona, Italy; Department of Clinical and Molecular Sciences, Section of Experimental Pathology, Marche Polytechnic University, I-60020, Ancona, Italy.
Neurosci Res. 2020 Feb;151:15-30. doi: 10.1016/j.neures.2019.02.005. Epub 2019 Feb 20.
The postnatal development of nitric oxide (NO)-producing intracallosal neurons was studied in rats by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry from postnatal day 0 (P0) to P30. NADPH-d-positive neurons (NADPH-d+) were detected already at P0, mainly in the rostral region of the corpus callosum (cc). Their location and the intensity of staining allowed them to be classified as type I NO-producing neurons. At P0, tufts of intensely labeled fibers, probably corresponding to the callosal septa described in the monkey and human cc, entered the ventral cc region and reached its dorsal portion. From P5, cell bodies and dendrites were often associated to blood vessels. The number of intracallosal NADPH-d+ rose in the first postnatal days to peak at P5, it declined until P10, and then remained almost constant until P30. Their size increased from P0 to P30, dramatically so (>65%) from P0 to P15. From P10 onward their distribution was adult-like, i.e. NADPH-d+ were more numerous in the lateral and intermediate portions of the cc and diminished close to the midline. In conjunction with previous data, these findings indicate that intracallosal NADPH-d+ could have a role in callosal axon guidance, myelination, refinement processes, and callosal blood flow regulation.
用烟酰胺腺嘌呤二核苷酸磷酸（NADPH-d）组织化学方法，从出生后第 0 天（P0）到第 30 天（P30），研究了大鼠室间联络体内产生一氧化氮（NO）的神经元的产后发育。在 P0 时已经检测到 NADPH-d 阳性神经元（NADPH-d+），主要位于胼胝体（cc）的前区。它们的位置和染色强度使它们被分类为 I 型产生 NO 的神经元。在 P0 时，束状的强烈标记纤维，可能对应于猴和人 cc 中描述的胼胝体隔，进入 cc 的腹侧区域并到达其背侧部分。从 P5 开始，细胞体和树突经常与血管相关。室间联络体内的 NADPH-d+数量在出生后的头几天增加，在 P5 时达到峰值，然后下降到 P10，然后直到 P30 几乎保持不变。它们的大小从 P0 到 P30 增加，从 P0 到 P15 增加非常显著（>65%）。从 P10 开始，它们的分布类似于成人，即 NADPH-d+在 cc 的外侧和中间部分更多，而在中线附近减少。结合以前的数据，这些发现表明室间联络体内的 NADPH-d+可能在胼胝体轴突引导、髓鞘形成、细化过程和胼胝体血流调节中发挥作用。
