Department of Central Lab, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China; The Key Lab of Precision Diagnosis and Treatment in Hematologic Malignancies of Zhenjiang City, China.
Department of Hematology, Kunshan Third People's Hospital, KunShan, Jiangsu, China.
Biomed Pharmacother. 2019 Apr;112:108623. doi: 10.1016/j.biopha.2019.108623. Epub 2019 Feb 20.
Dysregulation of miR-378 has been found in diverse types of tumors as well as in leukemia. The role of miR-378 in chronic myeloid leukemia (CML) remains unclear. The aim of the study was to reveal the potential effects of miR-378 in the pathological process and progress in CML. Our results showed general level of miR-378 was significant higher in CML patients compared to controls. Overexpression of miR-378 dramatically promoted cell proliferation and drug-resistance. Additionally, apoptosis was inhibited in cells transfected with miR-378. More and bigger stem cell sphere formation was observed in miR-378 transfected cells. Furthermore, enhanced expression of miR-378 was associated with upregulation of stem-cell makers OCT4 and c-Myc. Further study validated that miR-378 inhibited the expression of FUS1. Our research demonstrated the oncogenic nature of miR-378 in CML, and might contribute to the progress of CML.
miR-378 的失调已在多种类型的肿瘤以及白血病中被发现。miR-378 在慢性髓性白血病（CML）中的作用尚不清楚。本研究旨在揭示 miR-378 在 CML 病理过程和进展中的潜在作用。
我们的研究结果表明，与对照组相比，CML 患者的 miR-378 总体水平显著升高。miR-378 的过表达显著促进了细胞增殖和耐药性。此外，转染 miR-378 的细胞中凋亡受到抑制。转染 miR-378 的细胞中观察到更多更大的干细胞球形成。此外，miR-378 的高表达与干细胞标志物 OCT4 和 c-Myc 的上调有关。进一步的研究验证了 miR-378 抑制了 FUS1 的表达。
我们的研究表明 miR-378 在 CML 中具有致癌性，可能有助于 CML 的进展。
