miR-378 通过增强慢性髓性白血病 K562 细胞的干细胞特性促进细胞增殖并抑制细胞凋亡。
MiR-378 promoted cell proliferation and inhibited apoptosis by enhanced stem cell properties in chronic myeloid leukemia K562 cells.
机构信息
Department of Central Lab, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China; The Key Lab of Precision Diagnosis and Treatment in Hematologic Malignancies of Zhenjiang City, China.
Department of Hematology, Kunshan Third People's Hospital, KunShan, Jiangsu, China.
出版信息
Biomed Pharmacother. 2019 Apr;112:108623. doi: 10.1016/j.biopha.2019.108623. Epub 2019 Feb 20.
Dysregulation of miR-378 has been found in diverse types of tumors as well as in leukemia. The role of miR-378 in chronic myeloid leukemia (CML) remains unclear. The aim of the study was to reveal the potential effects of miR-378 in the pathological process and progress in CML. Our results showed general level of miR-378 was significant higher in CML patients compared to controls. Overexpression of miR-378 dramatically promoted cell proliferation and drug-resistance. Additionally, apoptosis was inhibited in cells transfected with miR-378. More and bigger stem cell sphere formation was observed in miR-378 transfected cells. Furthermore, enhanced expression of miR-378 was associated with upregulation of stem-cell makers OCT4 and c-Myc. Further study validated that miR-378 inhibited the expression of FUS1. Our research demonstrated the oncogenic nature of miR-378 in CML, and might contribute to the progress of CML.
miR-378 的失调已在多种类型的肿瘤以及白血病中被发现。miR-378 在慢性髓性白血病（CML）中的作用尚不清楚。本研究旨在揭示 miR-378 在 CML 病理过程和进展中的潜在作用。
我们的研究结果表明，与对照组相比，CML 患者的 miR-378 总体水平显著升高。miR-378 的过表达显著促进了细胞增殖和耐药性。此外，转染 miR-378 的细胞中凋亡受到抑制。转染 miR-378 的细胞中观察到更多更大的干细胞球形成。此外，miR-378 的高表达与干细胞标志物 OCT4 和 c-Myc 的上调有关。进一步的研究验证了 miR-378 抑制了 FUS1 的表达。
我们的研究表明 miR-378 在 CML 中具有致癌性，可能有助于 CML 的进展。
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