School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Infinitus Chinese Herbal Immunity Research Centre, Guangzhou 510663, China; School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Biomed Pharmacother. 2019 Apr;112:108682. doi: 10.1016/j.biopha.2019.108682. Epub 2019 Feb 20.
Immunity due to immune balance contributes to disease prevention and treatment. Radix Codonopsis polysaccharide (RCP) is isolated from the root of the Chinese herb Codonopsis pilosula. Previous studies have indicated that RCP has immunomodulatory activities; however, the effects of RCP on immunity, especially immune balance, are still largely unknown. In the present study, we investigated the effects of RCP on T-cell balance in mice. The mice were pretreated intragastrically with or without RCP for 15 days and injected with hydrocortisone on days 10-15 to disturb the immune system. The spleen and thymus were weighed and used to calculate immune organ indexes. The percentages of CD4 T cells, CD8 T cells, Th1 cells, Th2 cells, regulatory T cells (Tregs) and Th17 cells in peripheral blood were assayed by flow cytometry. Pro-inflammatory and anti-inflammatory cytokines, TNF-α, IL-1β and IL-10, in serum were determined by enzyme-linked immunosorbent assay (ELISA) kits. The results showed that RCP pretreatment could maintain the homeostasis of CD8 T cells, Tregs, Th17 cells, TNF-α, IL-1β and IL-10 in hydrocortisone-treated mice. Furthermore, RCP pretreatment maintained the immune balance of CD4/CD8 T cells, Th1/Th2 cells, Tregs/Th17 cells, IL-10/TNF-α and IL-10/IL-1β. Taken together, RCP pretreatment had beneficial effects on the maintenance of T-cell balance against hydrocortisone disturbance in mice and potential to be developed into novel functional food.
免疫平衡介导的免疫有助于疾病的预防和治疗。党参多糖（RCP）是从党参的根部提取的。先前的研究表明 RCP 具有免疫调节活性；然而，RCP 对免疫的影响，特别是免疫平衡的影响，在很大程度上仍不清楚。在本研究中，我们研究了 RCP 对小鼠 T 细胞平衡的影响。小鼠经灌胃预处理 15 天，用或不用 RCP，第 10-15 天注射氢化可的松以扰乱免疫系统。称重脾脏和胸腺，并用于计算免疫器官指数。通过流式细胞术测定外周血中 CD4 T 细胞、CD8 T 细胞、Th1 细胞、Th2 细胞、调节性 T 细胞（Tregs）和 Th17 细胞的百分比。用酶联免疫吸附试验（ELISA）试剂盒测定血清中促炎和抗炎细胞因子 TNF-α、IL-1β 和 IL-10。结果表明，RCP 预处理可维持氢化可的松处理小鼠中 CD8 T 细胞、Tregs、Th17 细胞、TNF-α、IL-1β 和 IL-10 的内稳态。此外，RCP 预处理维持了 CD4/CD8 T 细胞、Th1/Th2 细胞、Tregs/Th17 细胞、IL-10/TNF-α 和 IL-10/IL-1β 的免疫平衡。总之，RCP 预处理对维持 T 细胞平衡具有有益作用，可防止氢化可的松对小鼠的干扰，有望开发成新型功能性食品。
