Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, PR China.
Int J Biol Macromol. 2020 Oct 1;160:814-822. doi: 10.1016/j.ijbiomac.2020.05.149. Epub 2020 May 21.
Codonopsis pilosula polysaccharide (CPPS) and selenizing CPPS (sCPPS) were prepared and identified by a combination of chemical and instrumental analysis. Their immune modulation activities were compared by lymphocyte proliferation and flowcytometry tests in vitro or serum antibody responses and cytokines with immunization against OVA mice in vivo. The results showed that the sCPPS was successfully modified in selenylation. In vitro, the sCPPS were more effective compared with CPPS in promoting lymphocyte proliferation synergistically with PHA or LPS and increasing the ratio of CD4+ to CD8 + T cells. In vivo, sCPPS could significantly raised IgG, IgM, IFN-γ, IL-2 and IL-4 contents in the serum of mouse against OVA in comparison with CPPS. These results indicate that selenylation modification can enhance the immune modulation activitives of CPPS. sCPPS would be as a component drug of new-type immunoenhancer.
党参多糖（CPPS）和硒化 CPPS（sCPPS）通过化学和仪器分析相结合进行了制备和鉴定。通过体外淋巴细胞增殖和流式细胞术试验或体内免疫 OVA 小鼠的血清抗体反应和细胞因子比较了它们的免疫调节活性。结果表明，sCPPS 成功地进行了硒化修饰。体外，sCPPS 比 CPPS 更有效地促进淋巴细胞增殖，与 PHA 或 LPS 协同作用，并增加 CD4+到 CD8+T 细胞的比例。体内，sCPPS 可显著提高 CPPS 相比，小鼠针对 OVA 的血清 IgG、IgM、IFN-γ、IL-2 和 IL-4 含量。这些结果表明，硒化修饰可以增强 CPPS 的免疫调节活性。sCPPS 将作为新型免疫增强剂的成分药物。
