Feng Haibo, Yang Jie, Zhi Hui, Hu Xin, Yang Yan, Zhang Linzi, Liu Qianqian, Feng Yangyang, Wu Daiyan, Li Hangyu
College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.
Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, China.
Pharmaceutics. 2021 Sep 1;13(9):1384. doi: 10.3390/pharmaceutics13091384.
In this investigation, to maximize the desired immunoenhancement effects of PsEUL and stimulate an efficient humoral and cellular immune response against an antigen, PsEUL and the model antigen ovalbumin (OVA) were coupled using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) reaction to yield a novel delivery system (PsEUL-OVA). The physicochemical characteristics and immune regulation effects of this new system were investigated. We found the yield of this EDC method to be 46.25%. In vitro, PsEUL-OVA (200 μg mL) could enhance macrophage proliferation and increase their phagocytic efficiency. In vivo, PsEUL-OVA could significantly increase the levels of OVA-specific antibody (IgG, IgG1, IgG2a, and IgG2b) titers and cytokine (IL-2, IL-4, IL-6, IFN-γ) levels. Additionally, it could activate T lymphocytes and facilitate the maturation of dendritic cells (DCs). These findings collectively suggested that PsEUL-OVA induced humoral and cellular immune responses by promoting the phagocytic activity of macrophages and DCs. Taken together, these results revealed that PsEUL-OVA had the potential to improve immune responses and provide a promising theoretical basis for the design of a novel delivery system.
在本研究中，为了最大化紫松果菊乙醇提取物（PsEUL）的预期免疫增强作用，并刺激针对抗原的有效体液免疫和细胞免疫反应，使用N-(3-二甲基氨基丙基)-N'-乙基碳二亚胺盐酸盐（EDC）反应将PsEUL与模型抗原卵清蛋白（OVA）偶联，以产生一种新型递送系统（PsEUL-OVA）。对该新系统的理化特性和免疫调节作用进行了研究。我们发现这种EDC方法的产率为46.25%。在体外，PsEUL-OVA（200μg/mL）可增强巨噬细胞增殖并提高其吞噬效率。在体内，PsEUL-OVA可显著提高OVA特异性抗体（IgG、IgG1、IgG2a和IgG2b）滴度水平以及细胞因子（IL-2、IL-4、IL-6、IFN-γ）水平。此外，它可激活T淋巴细胞并促进树突状细胞（DCs）成熟。这些发现共同表明，PsEUL-OVA通过促进巨噬细胞和DCs的吞噬活性诱导体液免疫和细胞免疫反应。综上所述，这些结果表明PsEUL-OVA具有改善免疫反应的潜力，并为新型递送系统的设计提供了有前景的理论基础。
