Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad 2001, Col. Chamilpa, C. P. 62210 Cuernavaca, Morelos, Mexico.
Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos. Av. Universidad 2001, Col. Chamilpa, C.P. 62210 Cuernavaca, Morelos, Mexico.
Food Chem. 2019 Jul 1;285:204-212. doi: 10.1016/j.foodchem.2019.01.130. Epub 2019 Jan 31.
Prebiotic fructooligosaccharides (FOS) are currently obtained by enzymatic reaction with fructosyltransferases (FTFs) using sucrose as both donor and acceptor. In these reactions glucose results as the most abundant by-product, arising from each fructosyl transfer event and, together with fructose, because of the inherent hydrolytic activity of the FTFs. As FOS are mainly used as prebiotic in nutraceutical foods, the reduction or total elimination of monosaccharides is required. In this work the selective elimination of monosaccharides from a synthetic FOS mixture was achieved through the selective complexation of glucose and fructose with phenyl boronic acid (PBAc) followed by ethyl-acetate extraction. The process was applied to a complex mixture of FOS obtained in an enzymatic synthesis reaction containing 40% glucose, 15.8% fructose and 35% of FOS, elimination of the sugars was achieved through 3:1 molar reactions, resulting in a levan-type FOS product with 97% purity.
目前，低聚果糖（FOS）是通过蔗糖作为供体和受体，利用果糖基转移酶（FTFs）的酶反应获得的。在这些反应中，葡萄糖是最丰富的副产物，每个果糖基转移事件都会产生葡萄糖，并且由于 FTFs 的固有水解活性，果糖也会产生。由于 FOS 主要用作营养食品中的益生元，因此需要减少或完全消除单糖。在这项工作中，通过苯硼酸（PBAc）与葡萄糖和果糖的选择性络合，然后用乙酸乙酯萃取，从合成的 FOS 混合物中选择性地去除单糖。该工艺应用于通过酶合成反应获得的 FOS 复杂混合物中，该反应含有 40%的葡萄糖、15.8%的果糖和 35%的 FOS，通过 3:1 摩尔反应实现了糖的去除，得到了 97%纯度的莱鲍迪甙 FOS 产物。
