Li Jihong, Song Jinlin, Meng Di, Yi Yin, Zhang Ting, Shu Yu, Wu Xiaohong
Stomatological Hospital of Chongqing Medical University, No. 426 Songshibei Road, Yubei District, Chongqing 401147, China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
Regen Biomater. 2023 Feb 20;10:rbad006. doi: 10.1093/rb/rbad006. eCollection 2023.
Repairing osteoporotic bone defects is still a major clinical challenge. Recent studies have revealed that immune response is also essential in osteogenesis. The intrinsic inflammatory response of the host, especially the M1/M2 polarization status and inflammatory secretory function of macrophages, can directly affect osteogenic differentiation. Therefore, in this study, an electrospun naringin-loaded microspheres/sucrose acetate isobutyrate (Ng-m-SAIB) system was constructed to investigate its effect on the polarization of macrophage and osteoporotic bone defects. The results of both and experiments showed that Ng-m-SAIB had good biocompatibility and could promote the polarization of macrophage toward M2, thereby forming a favorable microenvironment for osteogenesis. The animal experiments also showed that Ng-m-SAIB could promote the osteogenesis of critical size defects in the skull of the osteoporotic model mouse (the senescence-accelerated mouse-strain P6). Together, these results collectively suggested that Ng-m-SAIB might be a promising biomaterial to treat osteoporotic bone defects with favorable osteo-immunomodulatory effects.
修复骨质疏松性骨缺损仍是一项重大的临床挑战。最近的研究表明，免疫反应在骨生成中也至关重要。宿主的固有炎症反应，尤其是巨噬细胞的M1/M2极化状态和炎症分泌功能，可直接影响成骨分化。因此，在本研究中，构建了一种电纺载柚皮苷微球/醋酸异丁酸蔗糖酯（Ng-m-SAIB）体系，以研究其对巨噬细胞极化和骨质疏松性骨缺损的影响。体内和体外实验结果均表明，Ng-m-SAIB具有良好的生物相容性，可促进巨噬细胞向M2极化，从而形成有利于成骨的微环境。动物实验还表明，Ng-m-SAIB可促进骨质疏松模型小鼠（衰老加速小鼠品系P6）颅骨临界尺寸缺损的成骨。综上所述，这些结果共同表明，Ng-m-SAIB可能是一种有前景的生物材料，具有良好的骨免疫调节作用，可用于治疗骨质疏松性骨缺损。
