Li Ping, Yang Hong, Wang Lu, Liu Haoju, Huo Heqiang, Zhang Chengjun, Liu Aizhong, Zhu Andan, Hu Jinyong, Lin Yongjun, Liu Li
Key Laboratory for Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Genet. 2019 Feb 8;10:55. doi: 10.3389/fgene.2019.00055. eCollection 2019.
In some plants, exposure to stress can induce a memory response, which appears to play an important role in adaptation to recurrent stress environments. However, whether rice exhibits drought stress memory and the molecular mechanisms that might underlie this process have remained unclear. Here, we ensured that rice drought memory was established after cycles of mild drought and re-watering treatment, and studied gene expression by whole-transcriptome strand-specific RNA sequencing (ssRNA-seq). We detected 6,885 transcripts and 238 lncRNAs involved in the drought memory response, grouped into 16 distinct patterns. Notably, the identified genes of dosage memory generally did not respond to the initial drought treatment. Our results demonstrate that stress memory can be developed in rice under appropriate water deficient stress, and lncRNA, DNA methylation and endogenous phytohormones (especially abscisic acid) participate in rice short-term drought memory, possibly acting as memory factors to activate drought-related memory transcripts in pathways such as photosynthesis and proline biosynthesis, to respond to the subsequent stresses.
在一些植物中，暴露于胁迫条件下可诱导记忆反应，这一反应似乎在适应反复出现的胁迫环境中发挥重要作用。然而，水稻是否表现出干旱胁迫记忆以及这一过程背后的分子机制仍不清楚。在此，我们确保在轻度干旱和复水处理循环后建立水稻干旱记忆，并通过全转录组链特异性RNA测序（ssRNA-seq）研究基因表达。我们检测到6885个转录本和238个lncRNA参与干旱记忆反应，分为16种不同模式。值得注意的是，所鉴定的剂量记忆基因通常对初始干旱处理无反应。我们的结果表明，在适当的水分亏缺胁迫下，水稻能够形成胁迫记忆，lncRNA、DNA甲基化和内源植物激素（尤其是脱落酸）参与水稻短期干旱记忆，可能作为记忆因子激活光合作用和脯氨酸生物合成等途径中与干旱相关的记忆转录本，以应对后续胁迫。
