Department of Respiratory Medicine, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin, China.
Department of Radiology, Yanbian University Hospital, YanJi 133002, Jilin, China.
Biomed Res Int. 2019 Jan 20;2019:7823761. doi: 10.1155/2019/7823761. eCollection 2019.
This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both and . Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-, IL-4, IL-1, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-B signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-B, and Nrf2/HO-1 pathways.
本研究旨在探讨欧前胡素（IMP）对肥大细胞介导的过敏反应的影响，包括 和 。建立了被动皮肤过敏（PCA）模型。进行组织学检测以评估耳部组织学。ELISA 和 Western blot 分析用于检测相应细胞因子和信号通路蛋白的水平。IMP 以剂量依赖性方式降低 PCA 模型中伊文思蓝的泄漏和耳部厚度，并减轻肥大细胞脱颗粒。此外，IMP 降低了 TNF-、IL-4、IL-1、IL-8 和 IL-13 的表达。此外，IMP 抑制了 Syk、Lyn、PLC-1 和 Gab2 的磷酸化水平，以及下游的 MAPK、PI3K/AKT 和 NF-B 信号通路。此外，IMP 通过 Nrf2/HO-1 途径抑制肥大细胞介导的过敏反应。IMP 通过抑制脱颗粒和降低肥大细胞中促炎细胞因子的表达水平来减轻过敏反应，涉及 PI3K/Akt、MAPK、NF-B 和 Nrf2/HO-1 途径。
