Weinstein R S, Banner B F, Kuszak J R, Thomas N J, Pauli B U
Dig Dis Sci. 1986 Feb;31(2 Suppl):115S-119S. doi: 10.1007/BF01309334.
The effect of intragastric misoprostol, a synthetic prostaglandin E1 methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 micrograms/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 micrograms/kg misoprostol (P greater than 0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.
采用透射电子显微镜和扫描电子显微镜研究了合成前列腺素E1甲酯类似物米索前列醇胃内给药对大鼠胃形态学的影响。成年雄性Sprague-Dawley大鼠接受剂量范围为50至1000微克/千克体重的米索前列醇，30分钟后处死。在所有剂量水平下，胃表面、胃小凹内以及胃腺内衬的上皮细胞内细胞器超微结构均保持正常。采用定量冷冻断裂电子显微镜检查米索前列醇对上皮细胞紧密连接（黏膜屏障的主要结构成分）的影响。对照组与接受1000微克/千克米索前列醇的动物之间，膜内纤维数量和紧密连接复合体深度无显著差异（P>0.05）。因此，胃上皮紧密连接超微结构的改变似乎不能解释米索前列醇的细胞保护作用。
