Al-Gburi Bashar Kadhim Hadi, Al-Sahaf Fadhil Hussein, Al-Fadhal Fadhal Abdulhusain, Del Monte Juan Pablo
1Department of Plant Protection, Faculty of Agriculture, Kufa University, Najaf, Iraq.
2Department of Agricultural Production, E.T.S.I.A.A.B., Polytechnic University of Madrid, Madrid, Spain.
Physiol Mol Biol Plants. 2019 Jan;25(1):253-261. doi: 10.1007/s12298-018-0630-4. Epub 2018 Dec 14.
This study aimed to detect the chemical compounds contained in seeds and shoot parts using the GC-MS following methanol extraction method, and also determining protein, total phenolic, total soluble carbohydrates, plant hormones and pigments for both seeds and shoot (flower and filament). The GC-MS analysis results showed six compounds are common between seed and shoot and the presence of different compounds in different plant parts including 24 compounds in seeds and 9 compounds in the shoot parts. The content of protein and carbohydrate in shoot (1.095% and 22.640 mg g respectively) were higher than in seeds and the total phenols content in seeds (11.094 mg g) was greatest than in shoot. In addition, there were differences between seeds and filaments parts in terms of concentration of pigments and free plant hormones, filaments were superior in total chlorophyll and carotene content (5.37 mg 100 g and 13.26 mg 100 g respectively). In seeds, ABA content was very high reaching 93.38 µM compared to other hormones, which had an effect on the seed dormancy of .
本研究旨在采用甲醇萃取法,通过气相色谱 - 质谱联用仪(GC - MS)检测种子和地上部分所含的化合物,同时测定种子和地上部分(花和花丝)的蛋白质、总酚、总可溶性碳水化合物、植物激素和色素。GC - MS分析结果表明,种子和地上部分共有六种化合物,且不同植物部位存在不同的化合物,其中种子中有24种化合物,地上部分有9种化合物。地上部分的蛋白质和碳水化合物含量(分别为1.095%和22.640毫克/克)高于种子,种子中的总酚含量(11.094毫克/克)高于地上部分。此外,种子和花丝部分在色素和游离植物激素浓度方面存在差异,花丝的总叶绿素和胡萝卜素含量更高(分别为5.37毫克/100克和13.26毫克/100克)。在种子中,脱落酸(ABA)含量非常高,达到93.38微摩尔,相比其他激素,这对种子休眠有影响。