College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Tai'an City, 271018, Shandong Province, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Tai'an City, 271018, Shandong Province, China.
Biol Trace Elem Res. 2020 Jan;193(1):234-240. doi: 10.1007/s12011-019-01682-x. Epub 2019 Feb 25.
Cadmium (Cd), as one of the most toxic heavy metals, has become a widespread environmental contaminant and threats the food quality and safety. The protective effect of selenium (Se) on Cd-induced tissue lesion and cytotoxicity in chicken has been extensively reported. The objective of this study was to investigate the antagonistic effect of Se on Cd-induced damage of chicken pectoral muscles via analyzing the trace elements and amino acids profiles. Firstly, 19 trace elements contents were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that under Cd exposure, the contents of Cd, lead (Pb), mercury (Hg), aluminum (Al), and lithium (Li) were significantly elevated, and the contents of Se, iron (Fe), and chromium (Cr) were significantly reduced. However, supplementing Se significantly reversed the effects induced by Cd. Secondly, the amino acids contents were detected by L-8900 automatic amino acid analyzer. The results showed that supplementing Se increased significantly Cd-induced decrease of valine (Val), leucine (Leu), arginine (Arg), and proline (Pro). Thirdly, the results of principal component analysis (PCA) showed that cobalt (Co), manganese (Mn), silicium (Si), and Pro may play special roles in response to the process of Se antagonizes Cd-induced damage of pectoral muscles in chickens. In summary, these results indicated that different trace elements and amino acids possessed and exhibited distinct responses to suffer from Se and/or Cd in chicken pectoral muscles. Notably, Se alleviated Cd-induced adverse effects by regulating trace elements and amino acids profiles in chicken pectoral muscles.
镉（Cd）作为最毒的重金属之一，已成为广泛存在的环境污染物，威胁着食品的质量和安全。大量研究表明，硒（Se）对鸡镉诱导的组织损伤和细胞毒性具有保护作用。本研究旨在通过分析微量元素和氨基酸谱，探讨硒对鸡胸肌镉损伤的拮抗作用。首先，采用电感耦合等离子体质谱法（ICP-MS）分析了 19 种微量元素含量。结果表明，在镉暴露下，镉、铅（Pb）、汞（Hg）、铝（Al）和锂（Li）的含量显著升高，硒（Se）、铁（Fe）和铬（Cr）的含量显著降低。然而，补充硒可显著逆转镉引起的变化。其次，采用 L-8900 自动氨基酸分析仪检测了氨基酸含量。结果表明，补充硒可显著增加镉诱导的缬氨酸（Val）、亮氨酸（Leu）、精氨酸（Arg）和脯氨酸（Pro）含量的降低。第三，主成分分析（PCA）结果表明，钴（Co）、锰（Mn）、硅（Si）和 Pro 可能在硒拮抗鸡胸肌镉损伤过程中发挥特殊作用。综上所述，这些结果表明，不同的微量元素和氨基酸在鸡胸肌中对硒和/或镉的作用具有不同的响应。值得注意的是，硒通过调节鸡胸肌中微量元素和氨基酸谱缓解了镉引起的不良反应。
