正常大鼠乳腺组织和二甲基苯并蒽诱导的大鼠乳腺肿瘤中的磷脂酶C活性。
Phospholipase C activity in normal rat mammary tissues and in DMBA-induced rat mammary tumors.
作者信息
Rillema J A
出版信息
Proc Soc Exp Biol Med. 1986 Mar;181(3):450-3. doi: 10.3181/00379727-181-3-rc1.
Employing phosphatidylinositol as the substrate, phospholipase C (PLC) activity was measured in various cellular fractions derived from DMBA-induced rat mammary tumors and mammary tissues from 12-14 day pregnant rats. In the 2,000g pellet, 10,000g pellet, 100,000g pellet and 100,000g supernatant fractions, PLC activity was more than 5 fold higher in the fractions derived from the neoplastic tissues. This was true whether PLC activity was expressed on the basis of protein content or 5' nucleotidase activities.
以磷脂酰肌醇作为底物，在源自二甲基苯并蒽（DMBA）诱导的大鼠乳腺肿瘤以及来自怀孕12 - 14天大鼠的乳腺组织的各种细胞组分中测量了磷脂酶C（PLC）活性。在2000g沉淀、10000g沉淀、100000g沉淀和100000g上清液组分中，源自肿瘤组织的组分中的PLC活性比其他组分高5倍以上。无论PLC活性是以蛋白质含量还是5'核苷酸酶活性为基础来表示，都是如此。
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