Rillema J A, Osmialowski E C, Linebaugh B E
Biochim Biophys Acta. 1980 Jan 18;617(1):150-5. doi: 10.1016/0005-2760(80)90232-5.
The activities of phospholipase A2 were compared in mammary glands from virgin and mid-pregnant rats and in 9,10-dimethyl-1,2-benzanthracene-induced rat mammary tumors. Enzyme activities were not different in the 150 000 x g pellet fractions of mammary gland homogenates from virgin and mid-pregnant rats, but enzyme activity in the 150 000 x g supernatant fraction was about twice as high in the homogenates from the mid-pregnant rat glands. Phospholipase A2 activities in the 150 000 x g pellet and supernatant fractions of homogenerates of growing tumor tissues were more than an order of magnitude higher than in the normal tissues. The elevated activity of phospholipase A2 in the tumor tissues may be related to their rapid rate of proliferation.
比较了未孕大鼠和妊娠中期大鼠乳腺以及9,10 - 二甲基 - 1,2 - 苯并蒽诱导的大鼠乳腺肿瘤中磷脂酶A2的活性。未孕大鼠和妊娠中期大鼠乳腺匀浆150000×g沉淀组分中的酶活性没有差异，但妊娠中期大鼠乳腺匀浆150000×g上清液组分中的酶活性约为未孕大鼠乳腺匀浆的两倍。生长中的肿瘤组织匀浆150000×g沉淀和上清液组分中的磷脂酶A2活性比正常组织高一个数量级以上。肿瘤组织中磷脂酶A2活性的升高可能与其快速增殖率有关。
