Health Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan; Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China.
Health Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Colloids Surf B Biointerfaces. 2019 May 1;177:496-505. doi: 10.1016/j.colsurfb.2019.02.026. Epub 2019 Feb 19.
Excellent biocompatibility and inflammatory regulation ability are essential to bone repair materials. Herein, Rod-like HAP with a diameter of 0.1 μm and Flake-like HAP with a width of 0.5-1 μm were synthesized by hydrothermal method, and then combined with two kinds of biomolecules, Icariin and Kaempferol. Two kinds of HAPs have similar crystal structure, but different zeta potentials and specific surface area. Rod-like HAP possesses stronger loading capacity and internalization efficiency than Flake-like one. in vitro inflammation assay reveals that HAP particles up-regulate the expression of IL-1β, TNF-α, IL-6, IL-10, IFN-γ and IL-2 cytokines. HAP particles loaded with Icariin or Kaempferol biomolecules up-regulate anti-inflammatory cytokines and down-regulate the expression of inflammatory cytokines.
良好的生物相容性和炎症调节能力是骨修复材料的关键。在此，我们通过水热法合成了直径为 0.1μm 的 Rod-like HAP 和宽度为 0.5-1μm 的 Flake-like HAP，并将其与两种生物分子淫羊藿苷和山奈酚结合。两种 HAP 具有相似的晶体结构，但具有不同的zeta 电位和比表面积。与 Flake-like HAP 相比，Rod-like HAP 具有更强的载药能力和内化效率。体外炎症实验表明，HAP 颗粒可上调 IL-1β、TNF-α、IL-6、IL-10、IFN-γ 和 IL-2 细胞因子的表达。负载有淫羊藿苷或山奈酚生物分子的 HAP 颗粒可上调抗炎细胞因子的表达，下调炎症细胞因子的表达。
