Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD198, INSERM 1095, Assistance Publique-Hôpitaux de Marseille, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Marseille, France.
Clinical Microbiology Department, Faculty of Medicine and Medical sciences, University of Balamand, Amioun, Lebanon.
Microbiologyopen. 2018 Oct;7(5):e00580. doi: 10.1002/mbo3.580. Epub 2018 Jun 13.
Using culturomics, a recently developed strategy based on diversified culture conditions for the isolation of previously uncultured bacteria, we isolated strain Marseille-P3296 from a fecal sample of a healthy pygmy female. A multiphasic approach, taxono-genomics, was used to describe the major characteristics of this anaerobic and gram-positive bacillus that is unable to sporulate and is not motile. The genome of this bacterium is 1,878,572 bp-long with a 57.94 mol% G + C content. On the basis of these characteristics and after comparison with its closest phylogenetic neighbors, we are confident that strain Marseille-P3296 (=CCUG 70328 = CSUR P3296) is the type strain of a novel species for which we propose the name Collinsella bouchesdurhonensis sp. nov.
使用文化组学，这是一种基于多样化培养条件的新兴策略，用于分离以前未培养的细菌，我们从一名健康的 pygmy 女性的粪便样本中分离出了马赛菌株 P3296。我们采用多相分类基因组学方法来描述这种不能形成孢子且不运动的厌氧革兰氏阳性杆菌的主要特征。该细菌的基因组长度为 1,878,572bp，G+C 含量为 57.94 mol%。基于这些特征，并与最接近的系统发育邻居进行比较，我们有信心马赛菌株 P3296（=CCUG 70328 = CSUR P3296）是一种新型物种的模式菌株，我们建议将其命名为 Collinsella bouchesdurhonensis sp. nov.。
