Pontificia Universidad Católica del Ecuador, Quito, Unidad de Investigaciones en Biomedicina, Zurita & Zurita Laboratorios, Quito, Servicio de Microbiología y Tuberculosis, Hospital Vozandes, Quito, Ecuador.
Pontificia Universidad Católica del Ecuador, Quito.
Int J Tuberc Lung Dis. 2019 Feb 1;23(2):166-173. doi: 10.5588/ijtld.18.0095.
The genetic diversity of in Quito, Ecuador is not well known.
To investigate mutations related to drug resistance and bacterial genotypes in strains in Ecuador.
This was a retrospective study of isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variable-number tandem repeats (MIRU-VNTR).
Isolates showed mutations in the B and G genes, and the A promoter. In B, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In G, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in G: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed.
This is the first report from Ecuador; it describes five new mutations in G and indicates that LAM is the most prevalent lineage.
厄瓜多尔基多的 遗传多样性尚不清楚。
研究厄瓜多尔 菌株中与耐药性相关的突变和细菌基因型。
这是一项对来自 104 名患者的 104 株分离株的回顾性研究。分离株对利福平（RMP）和/或异烟肼（INH）表型耐药。基因型采用 24 位位点分枝杆菌插入重复单位-可变数串联重复（MIRU-VNTR）确定。
分离株在 B 和 G 基因以及 A 启动子中显示突变。在 B 中，我们在密码子 511、513、514、515、516、526 和 531 处发现了 13 种遗传改变。46（44.2%）RMP 耐药分离株属于密码子 531。在 G 中，在密码子 296、312、314、315、322、324 和 351 处有 9 种遗传改变。53（51%）INH 耐药分离株属于密码子 315。在 G 中发现了五个以前未描述的突变：Thr324Ser、Thr314Ala、Ala312Pro、Trp351Stop 和 296 密码子缺失 G。以前观察到的主要突变中，拉丁美洲地中海（LAM）（33.7%）和加纳（30.8%）谱系最为常见。
这是厄瓜多尔的首次报告；它描述了 G 中的五个新突变，并表明 LAM 是最常见的谱系。
