Center for Hearing Research, Boys Town National Research Hospital, Omaha, NE, USA.
Department of Hearing & Speech, University of Kansas Medical Center, Kansas City, KS, USA.
Hear Res. 2019 Apr;375:53-65. doi: 10.1016/j.heares.2019.02.001. Epub 2019 Feb 15.
The cochlear microphonic, traditionally thought of as an indication of electrical current flow through hair cells, in conjunction with suppressing high-pass noise or tones, is a promising method of assessing the health of outer hair cells at specific locations along the cochlear partition. We propose that the electrical potential recorded from the round window in gerbils in response to low-frequency tones, which we call cochlear response (CR), contains significant responses from multiple cellular sources, which may expand its diagnostic purview. In this study, CR is measured in the gerbil and modeled to identify its contributing sources. CR was recorded via an electrode placed in the round window niche of sixteen Mongolian gerbils and elicited with a 45 Hz tone burst embedded in 18 high-pass filtered noise conditions to target responses from increasing regions along the cochlear partition. Possible sources were modeled using previously-published hair cell and auditory nerve response data, and then weighted and combined using linear regression to produce a model response that fits closely to the mean CR waveform. The significant contributing sources identified by the model are outer hair cells, inner hair cells, and the auditory nerve. We conclude that the low-frequency CR contains contributions from several cellular sources.
耳蜗微音器，传统上被认为是毛细胞中电流流动的指示，与抑制高通噪声或音调相结合，是评估耳蜗分区特定位置外毛细胞健康状况的一种很有前途的方法。我们提出，在响应低频音调时从沙鼠的圆窗记录的电势能，我们称之为耳蜗反应（CR），包含来自多个细胞源的显著反应，这可能扩大其诊断范围。在这项研究中，我们在沙鼠中测量了 CR 并对其进行建模以确定其贡献源。通过放置在十六只蒙古沙鼠圆窗窝中的电极记录 CR，并通过嵌入在 18 种高通滤波噪声条件中的 45 Hz 短音脉冲来激发，以针对耳蜗分区中逐渐增加的区域的反应。使用先前发表的毛细胞和听神经反应数据对可能的源进行建模，然后使用线性回归对其进行加权和组合，以产生与平均 CR 波形拟合良好的模型响应。模型确定的重要贡献源是外毛细胞、内毛细胞和听神经。我们得出结论，低频 CR 包含来自多个细胞源的贡献。
