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RNAi 依赖性抑制控制. 中的转座元件。

RNAi-dependent repression controls transposable elements in .

机构信息

Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.

出版信息

Genes Dev. 2019 Mar 1;33(5-6):348-364. doi: 10.1101/gad.320796.118. Epub 2019 Feb 26.

DOI:10.1101/gad.320796.118
PMID:30808657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6411011/
Abstract

RNAi and repression play evolutionarily conserved and often coordinated roles in transcriptional silencing. Here, we show that, in the protozoan , germline-specific internally eliminated sequences (IESs)-many related to transposable elements (TEs)-become transcriptionally activated in mutants deficient in the RNAi-dependent repression pathway. Germline TE mobilization also dramatically increases in these mutants. The transition from noncoding RNA (ncRNA) to mRNA production accompanies transcriptional activation of TE-related sequences and vice versa for transcriptional silencing. The balance between ncRNA and mRNA production is potentially affected by cotranscriptional processing as well as RNAi and repression. We posit that interplay between RNAi and repression is a widely conserved phenomenon, whose ancestral role is epigenetic silencing of TEs.

摘要

RNAi 和抑制在转录沉默中发挥着进化保守且常常协调一致的作用。在这里，我们表明，在原生动物中，生殖细胞特异性的内部消除序列（IESs）-许多与转座元件（TEs）相关-在 RNAi 依赖性抑制途径缺陷的突变体中变得转录激活。生殖细胞 TE 运动在这些突变体中也显著增加。从非编码 RNA（ncRNA）到 mRNA 产生的转变伴随着与 TE 相关序列的转录激活，反之亦然，转录沉默。ncRNA 和 mRNA 产生之间的平衡可能受到共转录加工以及 RNAi 和抑制的影响。我们假设，RNAi 和抑制之间的相互作用是一种广泛保守的现象，其古老的作用是对 TE 的表观遗传沉默。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/85383da7c16f/348f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/882d9c5bcbbe/348f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/349f756c4b25/348f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/a22242fd0ec2/348f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/32425be22d97/348f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/43c572597759/348f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/dc050609c04a/348f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/85383da7c16f/348f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/882d9c5bcbbe/348f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/349f756c4b25/348f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/a22242fd0ec2/348f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/32425be22d97/348f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/43c572597759/348f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/dc050609c04a/348f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a91/6411011/85383da7c16f/348f07.jpg

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