Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352;
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352.
Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3919-3928. doi: 10.1073/pnas.1815356116. Epub 2019 Feb 11.
Ebola virus disease (EVD) often leads to severe and fatal outcomes in humans with early supportive care increasing the chances of survival. Profiling the human plasma lipidome provides insight into critical illness as well as diseased states, as lipids have essential roles as membrane structural components, signaling molecules, and energy sources. Here we show that the plasma lipidomes of EVD survivors and fatalities from Sierra Leone, infected during the 2014-2016 Ebola virus outbreak, were profoundly altered. Focusing on how lipids are associated in human plasma, while factoring in the state of critical illness, we found that lipidome changes were related to EVD outcome and could identify states of disease and recovery. Specific changes in the lipidome suggested contributions from extracellular vesicles, viremia, liver dysfunction, apoptosis, autophagy, and general critical illness, and we identified possible targets for therapies enhancing EVD survival.
埃博拉病毒病 (EVD) 常导致人类出现严重且致命的后果，早期支持性治疗可增加生存机会。分析人类血浆脂质组可深入了解危重病以及疾病状态，因为脂质在作为膜结构成分、信号分子和能量来源方面具有重要作用。在这里，我们展示了塞拉利昂 2014-2016 年埃博拉病毒爆发期间感染 EVD 的幸存者和死亡者的血浆脂质组发生了深刻改变。我们关注的是脂质在人类血浆中如何相互关联，同时考虑到危重病的状态，我们发现脂质组的变化与 EVD 结果有关，并可以识别疾病和恢复状态。脂质组的特定变化表明与细胞外囊泡、病毒血症、肝功能障碍、细胞凋亡、自噬和一般危重病有关，我们确定了可能增强 EVD 生存的治疗目标。
