Wu Zong-Yen, Huang Yao-Ting, Chao Wen-Cheng, Ho Shu-Peng, Cheng Jan-Fang, Liu Po-Yu
US Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
J Adv Res. 2019 Jan 31;18:61-69. doi: 10.1016/j.jare.2019.01.011. eCollection 2019 Jul.
Antibiotic resistance in pathogens is a growing threat to human health. Of particular concern is resistance to carbapenem, which is an antimicrobial agent listed as critically important by the World Health Organization. With the global spread of carbapenem-resistant organisms, there is an urgent need for new treatment options. is an emerging pathogen found in marine environments throughout the world that has increasing resistance to carbapenem. The organism is also a possible antibiotic resistance reservoir in humans and in its natural habitat. The development of CRISPR/Cas9-based methods has enabled precise genetic manipulation. A number of attempts have been made to knock out resistance genes in various organisms. The study used a single plasmid containing CRISPR/Cas9 and recE/recT recombinase to reverse an antibiotic-resistant phenotype in and showed gene is essential for the carbapenem resistance. This result demonstrates a potential validation strategy for functional genome annotation in .
病原体中的抗生素耐药性对人类健康构成了日益严重的威胁。特别令人担忧的是对碳青霉烯类的耐药性，碳青霉烯类是世界卫生组织列为极为重要的抗菌剂。随着耐碳青霉烯类微生物在全球的传播，迫切需要新的治疗选择。 是一种在世界各地海洋环境中发现的新兴病原体，对碳青霉烯类的耐药性不断增强。这种微生物也是人类及其自然栖息地中可能的抗生素耐药性储存库。基于CRISPR/Cas9方法的发展使得精确的基因操作成为可能。已经进行了许多尝试来敲除各种生物体中的耐药基因。该研究使用了一个含有CRISPR/Cas9和recE/recT重组酶的单一质粒来逆转 中的抗生素耐药表型，并表明 基因对于碳青霉烯类耐药性至关重要。这一结果证明了 在功能基因组注释方面的一种潜在验证策略。
