Department of Computer Science and Information Engineering, National Chung Cheng University, Chia-Yi, Taiwan.
Department of Energy, Joint Genome Institute, Walnut Creek, CA, USA.
J Glob Antimicrob Resist. 2018 Sep;14:65-67. doi: 10.1016/j.jgar.2018.06.005. Epub 2018 Jun 19.
Shewanella algae is an environmental marine bacterium and an emerging opportunistic human pathogen. Moreover, there are increasing reports of strains showing multidrug resistance, particularly carbapenem-resistant isolates. Although S. algae has been found in bivalve shellfish aquaculture, there is very little genome-wide data on resistance determinants in S. algae from shellfish. The aim of this study was to determine the whole genome sequence of carbapenem-resistant S. algae strain AC isolated from small abalone in Taiwan.
Bacterial genomic DNA was sequenced using an Illumina MiSeq platform with 250-bp paired-end reads. De novo genome assembly was performed using Velvet v.1.2.07. The whole genome was annotated and several candidate genes for antimicrobial resistance were identified.
The genome size was calculated at 4751156bp, with a mean G+C content of 53.09%. A total of 4164 protein-coding sequences, 7 rRNAs, 85 tRNAs and 5 non-coding RNAs were identified. The genome contains genes associated with resistance to β-lactams, trimethoprim, tetracycline, colistin and quinolones. Multiple efflux pump genes were also detected.
Small abalone is a potential source of foodborne drug-resistant S. algae. The genome sequence of carbapenem-resistant S. algae strain AC isolated from small abalone will provide valuable information for further study of the dissemination of resistance genes at the human-animal interface.
海栖拉乌尔菌是一种环境海洋细菌，也是一种新兴的机会性病原体。此外，越来越多的报告显示，该菌具有多种药物耐药性，尤其是耐碳青霉烯类的分离株。尽管海栖拉乌尔菌已在贝类水产养殖中被发现，但有关贝类来源的海栖拉乌尔菌耐药决定因素的全基因组数据却很少。本研究旨在确定从台湾小型鲍中分离出的耐碳青霉烯类海栖拉乌尔菌 AC 株的全基因组序列。
使用 Illumina MiSeq 平台，以 250-bp 配对末端读取对细菌基因组 DNA 进行测序。使用 Velvet v.1.2.07 进行从头基因组组装。对全基因组进行注释，并鉴定了几个抗菌药物耐药候选基因。
基因组大小计算为 4751156bp，平均 G+C 含量为 53.09%。共鉴定出 4164 个蛋白编码序列、7 个 rRNA、85 个 tRNA 和 5 个非编码 RNA。该基因组包含与β-内酰胺类、甲氧苄啶、四环素、粘菌素和喹诺酮类药物耐药相关的基因。还检测到多个外排泵基因。
小型鲍是食源性病原体耐药海栖拉乌尔菌的潜在来源。从小型鲍中分离出的耐碳青霉烯类海栖拉乌尔菌 AC 株的基因组序列将为进一步研究人类-动物界面耐药基因的传播提供有价值的信息。
