Guo Xiaoyang, Jiang Jinghan, Liu Ying, Yu Lili, Chang Ruzhen, Guan Rongxia, Qiu Lijuan
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2021 Nov 18;12:791175. doi: 10.3389/fpls.2021.791175. eCollection 2021.
Salinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map salt tolerance-related genes. The two populations segregated as 1 (tolerant):2 (heterozygous):1 (sensitive), indicating a Mendelian segregation model. Using simple sequence repeat (SSR) markers together with the bulked segregant analysis (BSA) mapping strategy, we mapped a salt tolerance locus on chromosome 03 in F population Zhonghuang39×NY36-87 to a 98-kb interval, in which the known gene co-segregated with the salt tolerance locus. In the F population of Peking×NY36-87, the dominant salt tolerance-associated gene was detected and mapped on chromosome 18. We named this gene and fine mapped it to a 241-kb region. Time course analysis and a grafting experiment confirmed that Peking accumulated more Na in the shoot a root-based mechanism. These findings reveal that the tolerant wild soybean line NY36-87 contains salt tolerance-related genes and , providing genetic material and a novel locus for breeding salt-tolerant soybean.
盐度是影响大豆生长和产量的重要非生物胁迫因素。NY36 - 87是一种具有高耐盐性的野生大豆种质。在本研究中，使用了两个由NY36 - 87与两个盐敏感大豆品种中黄39和北京大豆衍生而来的F作图群体来定位耐盐相关基因。这两个群体的分离比例为1（耐盐）:2（杂合）:1（敏感），表明符合孟德尔分离模型。利用简单序列重复（SSR）标记结合混合分组分析法（BSA）作图策略，我们将中黄39×NY36 - 87的F群体中位于3号染色体上的一个耐盐位点定位到一个98 kb的区间内，其中已知基因与耐盐位点共分离。在北 京大豆×NY36 - 87的F群体中，检测到了显性耐盐相关基因并将其定位到18号染色体上。我们对该基因进行了命名并将其精细定位到一个241 kb的区域。时间进程分析和嫁接实验证实，北京大豆地上部积累了更多的钠，这是一种基于根部的机制。这些发现揭示了耐盐野生大豆品系NY36 - 87含有耐盐相关基因 和 ，为培育耐盐大豆提供了遗传材料和一个新的基因位点。
