Department of Environmental Sciences, UNISA Florida Campus, Florida, 1710, Republic of South Africa.
Department of Biological Sciences, University of Namibia, Private Bag 13301, 340 Mandume Ndemufayo Ave, Pionierspark, Windhoek, Namibia.
Int Microbiol. 2019 Jun;22(2):181-189. doi: 10.1007/s10123-018-00038-0. Epub 2018 Oct 26.
This study aimed to analyze the bacterial diversity in carwash effluents and to determine their potential for use in microbial degradation of environmental contaminants. Nine carwash effluent samples were collected for physicochemical and bacterial community diversity analysis using multi-digital probes and 16S rRNA gene amplicon sequencing respectively. The pH of all effluent samples was neutral to slightly alkaline. Oil and grease concentrations ranged from 15.3 to 49.7 mg/L. 16S gene amplicon sequencing of the nine samples produced 45,934-sequence reads, which translated to 13 bacterial phyla, 26 classes, and 43 genera. The most dominant phyla were Proteobacteria, Bacteroidetes, Firmicutes, and Fusobacteria. Canonical correspondence analysis (CCA) showed that the distribution of the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Verrucomicrobia was influenced by the presence of oil and grease, total petroleum hydrocarbons-gasoline range organics (GRO-TPH), and metals species (Pb, Cu, and Zn). The dominant bacterial genera found in the present study were previously proven to biodegrade hydrocarbons, and their presence in carwash effluents could bode well for in situ natural bioremediation of these contaminated sites.
本研究旨在分析洗车废水中的细菌多样性，并确定其在环境污染物微生物降解中的应用潜力。本研究分别采用多数字探针和 16S rRNA 基因扩增子测序对 9 个洗车废水样本进行理化性质和细菌群落多样性分析。所有废水样本的 pH 值均呈中性至微碱性。油和油脂浓度范围为 15.3 至 49.7mg/L。9 个样本的 16S 基因扩增子测序产生了 45934 条序列，可分为 13 个细菌门、26 个纲和 43 个属。最主要的门是变形菌门、拟杆菌门、厚壁菌门和梭杆菌门。典范对应分析（CCA）表明，门Proteobacteria、Bacteroidetes、Acidobacteria 和 Verrucomicrobia 的分布受油和油脂、总石油烃-汽油范围有机物（GRO-TPH）和金属种类（Pb、Cu 和 Zn）的影响。本研究中发现的优势细菌属以前被证明可以生物降解烃类，它们在洗车废水中的存在可能预示着这些污染场地的原位自然生物修复。
