自动细菌菌落计数器的批判性评估

A Critical Evaluation of Automatic Bacterial Colony Counters.

作者信息

Brodsky M H, Ciebin B W, Schiemann D A

机构信息

Ontario Ministry of Health, Laboratory Services Branch, Environmental Bacteriology. Box 9000, Terminal A, Toronto, Ontario M5W 1R5, Canada.

出版信息

J Food Prot. 1979 Feb;42(2):138-143. doi: 10.4315/0362-028X-42.2.138.

DOI:10.4315/0362-028X-42.2.138

PMID:30812341

Abstract

Four automatic colony counters (ACC), 3M Model 620 and Artek Models 480, 870 and 880, were evaluated for their precision and accuracy in counting bacterial colonies in pour-plates prepared using raw and pasteurized milk samples. The automatic colony counters were precise, labor saving devices. but not one of the ACC units approached our acceptability criterion that 90% of the ACC counts fall within ± 10% of the corresponding manual count. Some parameters of experimental design and instrument calibration which may significantly influence the response and performance of the automatic counters are discussed.

摘要

对4台自动菌落计数器（ACC）进行了评估，分别是3M公司的620型以及Artek公司的480型、870型和880型，以检测其在对用生牛奶和巴氏杀菌牛奶样本制备的倾注平板中的细菌菌落计数时的精密度和准确性。这些自动菌落计数器是精确且节省人力的设备。但是，没有一台ACC设备达到我们的可接受标准，即90%的ACC计数落在相应人工计数的±10%范围内。本文讨论了一些可能会显著影响自动计数器响应和性能的实验设计和仪器校准参数。